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TD (effects)Tj 36.83 0 TD (of)Tj 13.017 0 TD (mic)Tj 18.164 0 TD [ (r)21 (o)] TJ 10.485 0 TD (be)Tj 12.515 0 TD (s)Tj 8.437 0 TD (in agric)Tj 37.878 0 TD (u)Tj 6.254 0 TD (lture.)Tj /F2 12 Tf -314.209 -28.75 TD (Natio)Tj 29.231 0 TD [ (n)21 (al)-250 (S)63 (t)42 (a)21 (nd)42 (a)21 (rd)21 (s)] TJ /F3 12 Tf -29.235 -14.5 TD (The atoms)Tj 55.71 0 TD (and mo)Tj 38.28 0 TD (l)Tj 2.917 0 TD (ec)Tj 11.528 0 TD [ (ule)21 (s)] TJ 24.451 0 TD (on the earth cy)Tj 76.113 0 TD (cl)Tj 8.602 0 TD (e among the living and nonliving components)Tj 235.493 0 TD (of)Tj 12.767 0 TD (the)Tj -465.886 -14.5 TD (b)Tj 6.671 0 TD (io)Tj 9.598 0 TD (s)Tj 5.437 0 TD (phere.)Tj -21.771 -28.75 TD (Ener)Tj 22.938 0 TD [ (g)21 (y)] TJ 14.747 0 TD (flo)Tj 13.184 0 TD (ws)Tj 16.751 0 TD (through ec)Tj 54.82 0 TD (o)Tj 6.419 0 TD (sy)Tj 11.314 0 TD [ (s)21 (t)] TJ 9.263 0 TD (ems)Tj 24.081 0 TD 0.27 Tw (in one direction, from phot)Tj 0 Tw 136.63 0 TD (o)Tj 6.169 0 TD (synthetic)Tj 49.634 0 TD (or)Tj 10.485 0 TD (g)Tj 6.119 0 TD (anisms)Tj 39.535 0 TD (t)Tj 3.826 0 TD (o herbivor)Tj 52.07 0 TD [ (e)21 (s)-229 (to)] TJ -477.975 -14.25 TD (c)Tj 5.435 0 TD (arnivor)Tj 35.656 0 TD (es)Tj 14.53 0 TD (and decomposers.)Tj -55.55 -29 TD (Org)Tj 18.248 0 TD (anisms)Tj 39.535 0 TD (both cooperate and compete in ec)Tj 173.498 0 TD (o)Tj 6.169 0 TD (sy)Tj 11.314 0 TD (st)Tj 9.513 0 TD (ems. The interrelationship)Tj 133.565 0 TD (s)Tj 8.437 0 TD (and interdependencie)Tj 111.726 0 TD (s)Tj 8.437 0 TD (of)Tj -520.483 -14.25 TD (these or)Tj 41.848 0 TD (g)Tj 6.119 0 TD (anisms)Tj 39.535 0 TD [ (ma)21 (y)-250 (g)] TJ 30.215 0 TD (enerate ec)Tj 53.103 0 TD (o)Tj 6.169 0 TD (sy)Tj 11.314 0 TD (st)Tj 9.513 0 TD (ems)Tj 24.081 0 TD (that)Tj 23.264 0 TD (are s)Tj 24.982 0 TD (t)Tj 3.576 0 TD (ab)Tj 12.838 0 TD (l)Tj 2.917 0 TD (e for hundred)Tj 69.055 0 TD (s)Tj 8.437 0 TD (or thou)Tj 36.897 0 TD (s)Tj 5.437 0 TD (and)Tj 19.342 0 TD (s)Tj 8.687 0 TD (of)Tj 12.767 0 TD [ (y)21 (e)] TJ 11.472 0 TD (ars.)Tj /F2 12 Tf -461.446 -28.75 TD (Ba)Tj 13.045 0 TD [ (c)42 (kg)42 (ro)21 (u)21 (n)] TJ 45.853 0 TD (d & Definitio)Tj 70.681 0 TD [ (n)42 (s)] TJ /F3 12 Tf -129.715 -14.5 TD (Sof)Tj 16.998 0 TD [ (t)-250 (r)] TJ 10.893 0 TD (ot)Tj 12.995 0 TD (of)Tj 13.017 0 TD (fl)Tj 6.765 0 TD (e)Tj 5.844 0 TD (sh)Tj 12.381 0 TD [ (y)-208 (veg)] TJ 25.834 0 TD (etab)Tj 22.508 0 TD [ (l)-21 (e)21 (s)] TJ 17.947 0 TD (and ornamental)Tj 83.298 0 TD (pl)Tj 9.564 0 TD (ants)Tj 24.945 0 TD [ (i)-21 (s)-229 (c)] TJ 17.05 0 TD (aused b)Tj 40.499 0 TD (y)Tj 8.628 0 TD (the bacterium Er)Tj 83.871 0 TD (w)Tj 8.314 0 TD (inia carot)Tj 47.421 0 TD (ov)Tj 11.912 0 TD (or)Tj 10.235 0 TD [ (a)-229 (v)] TJ 14.41 0 TD (ar)Tj 9.983 0 TD (.)Tj -515.116 -14.5 TD (The bacterium i)Tj 78.7 0 TD (s)Tj 8.437 0 TD (a Gram-ne)Tj 52.558 0 TD (g)Tj 6.119 0 TD (ative rod, appr)Tj 74.81 0 TD [ (o)42 (x)] TJ 11.832 0 TD (imately)Tj 40.111 0 TD [ (0)21 (.)] TJ 9.613 0 TD (7x1.5um and h)Tj 73.288 0 TD [ (a)21 (s)] TJ 14.354 0 TD (peritrichou)Tj 55.859 0 TD (s)Tj 8.437 0 TD (fl)Tj 6.515 0 TD (ag)Tj 12.536 0 TD (el)Tj 9.261 0 TD (l)Tj 2.917 0 TD (a. It)Tj 25.277 0 TD [ (i)21 (s)] TJ 11.865 0 TD (non-)Tj -502.664 -14.25 TD (s)Tj 5.937 0 TD (pore forming and fac)Tj 105.619 0 TD (u)Tj 6.504 0 TD (ltatively)Tj 43.297 0 TD (anaer)Tj 28.76 0 TD (ob)Tj 12.84 0 TD (ic. It)Tj 27.973 0 TD (gr)Tj 10.436 0 TD [ (o)21 (ws)-229 (w)] TJ 31.234 0 TD (el)Tj 9.261 0 TD (l)Tj 5.917 0 TD (in nutrient)Tj 55.939 0 TD (ag)Tj 12.286 0 TD (ar and nutrient)Tj 79.038 0 TD (broth but)Tj 50.646 0 TD (not)Tj 19.511 0 TD (abo)Tj 19.007 0 TD (ve)Tj -524.163 -14.5 TD (36 degree)Tj 51.037 0 TD (s)Tj 8.687 0 TD (C. The sof)Tj 53.172 0 TD [ (t)-208 (r)] TJ 10.393 0 TD (ot)Tj 13.245 0 TD (b)Tj 6.421 0 TD (acteria can gr)Tj 69.172 0 TD (o)Tj 6.419 0 TD (w and are active o)Tj 92.218 0 TD (v)Tj 5.243 0 TD (er a ran)Tj 39.012 0 TD (g)Tj 6.369 0 TD (e of)Tj 21.578 0 TD (t)Tj 3.826 0 TD (emperatur)Tj 52.766 0 TD (es)Tj 14.78 0 TD (from 3-35)Tj -454.228 -14.25 TD (degree)Tj 35.126 0 TD [ (s)-229 (C)-229 (b)] TJ 24.695 0 TD (ut)Tj 13.08 0 TD (are k)Tj 25.389 0 TD [ (il)21 (l)] TJ 9.263 0 TD (ed b)Tj 22.142 0 TD [ (y)-229 (e)] TJ 14.472 0 TD (xtended expo)Tj 69.302 0 TD (s)Tj 5.437 0 TD (ure at)Tj 32.625 0 TD (about)Tj 32.337 0 TD (50 degree)Tj 50.703 0 TD (s)Tj 8.687 0 TD (C.)Tj -343.278 -29 TD (Sof)Tj 16.998 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD (oc)Tj 11.854 0 TD (c)Tj 5.185 0 TD (ur)Tj 10.57 0 TD [ (s)-229 (w)] TJ 16.751 0 TD (orldwide wherever fl)Tj 103.924 0 TD (e)Tj 6.094 0 TD (sh)Tj 12.131 0 TD [ (y)-250 (s)21 (t)] TJ 18.141 0 TD (or)Tj 10.235 0 TD (ag)Tj 12.536 0 TD (e ti)Tj 15.567 0 TD [ (s)-21 (s)] TJ 11.373 0 TD (ue)Tj 12.348 0 TD (s)Tj 8.687 0 TD (of)Tj 12.767 0 TD [ (ve)21 (g)] TJ 17.706 0 TD (etab)Tj 22.508 0 TD (les)Tj 17.697 0 TD (and ornamental)Tj 80.548 0 TD (s)Tj 8.437 0 TD (are found.)Tj -455.626 -14.25 TD (P)Tj 6.277 0 TD (otatoes, carrots, and onion)Tj 137.532 0 TD (s)Tj 8.687 0 TD (are among the mo)Tj 92.587 0 TD (st)Tj 12.263 0 TD (affected )Tj 44.188 0 TD (veg)Tj 17.956 0 TD (etab)Tj 22.508 0 TD [ (l)21 (es)] TJ 17.697 0 TD (along with tomato and cucumber)Tj 167.332 0 TD (.)Tj -527.116 -14.5 TD (Iri)Tj 10.209 0 TD [ (s)-229 (b)-21 (ul)21 (b)21 (s)] TJ 39.886 0 TD (\(and simi)Tj 47.444 0 TD (l)Tj 2.917 0 TD (ar ornamentals\) are of)Tj 112.707 0 TD (t)Tj 3.826 0 TD (en affected. The dise)Tj 108.796 0 TD (a)Tj 5.917 0 TD 0.22 Tw (se can be found on c)Tj 0 Tw 105.317 0 TD (r)Tj 4.066 0 TD (op)Tj 12.566 0 TD (s)Tj 8.937 0 TD (in the field, in)Tj -462.704 -14.25 TD (tran)Tj 20.325 0 TD (s)Tj 5.687 0 TD (it, and in s)Tj 54.074 0 TD (t)Tj 3.826 0 TD (or)Tj 10.235 0 TD (ag)Tj 12.536 0 TD (e or during marketing r)Tj 116.922 0 TD (esu)Tj 18.284 0 TD (lting in gr)Tj 48.873 0 TD (e)Tj 5.844 0 TD (at)Tj 12.993 0 TD (economic)Tj 51.796 0 TD (lo)Tj 9.336 0 TD (s)Tj 5.687 0 TD (ses. Sof)Tj 43.535 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD (c)Tj 5.185 0 TD (ause)Tj 24.451 0 TD (s)Tj 8.687 0 TD (gr)Tj 10.186 0 TD (e)Tj 6.094 0 TD (ater)Tj -498.097 -14.5 TD (t)Tj 3.826 0 TD (otal)Tj 22.079 0 TD (lo)Tj 9.586 0 TD (ss)Tj 14.373 0 TD (of)Tj 12.517 0 TD (produce than an)Tj 83.396 0 TD (y)Tj 8.378 0 TD (other bacterial)Tj 77.589 0 TD (dise)Tj 21.368 0 TD (a)Tj 6.167 0 TD (se.)Tj -259.336 -28.75 TD (The sof)Tj 37.014 0 TD [ (t)-250 (r)] TJ 10.893 0 TD (ot)Tj 12.995 0 TD (b)Tj 6.671 0 TD (acterium enter)Tj 74.135 0 TD [ (s)-250 (p)21 (l)] TJ 18.001 0 TD (ant)Tj 19.509 0 TD (ti)Tj 6.755 0 TD [ (s)-21 (s)] TJ 11.373 0 TD (ue)Tj 12.348 0 TD (s)Tj 8.687 0 TD (primarily)Tj 48.4 0 TD (through wounds, of)Tj 99.817 0 TD (t)Tj 3.826 0 TD (en c)Tj 20.762 0 TD (re)Tj 10.16 0 TD (ated b)Tj 31.885 0 TD (y)Tj 8.628 0 TD (insect)Tj 33.485 0 TD (f)Tj 3.348 0 TD (eeding or)Tj -478.636 -14.5 TD (brui)Tj 20.17 0 TD (s)Tj 5.687 0 TD (ing at)Tj 31.775 0 TD (h)Tj 6.694 0 TD (ar)Tj 10.483 0 TD [ (ve)21 (st)] TJ 20.85 0 TD (. Insects)Tj 48.527 0 TD (and water are effective in spr)Tj 147.981 0 TD (ea)Tj 12.011 0 TD 0.234 Tw (ding the bacterium. Once in the p)Tj 0 Tw 173.552 0 TD (l)Tj 3.167 0 TD (ant)Tj 19.259 0 TD (ti)Tj 7.005 0 TD (ss)Tj 11.123 0 TD (ue,)Tj -518.21 -14.25 TD (the bacterium produc)Tj 109.273 0 TD (es)Tj 14.53 0 TD (inc)Tj 15.129 0 TD [ (re)21 (as)] TJ 21.514 0 TD (ing amounts)Tj 66.451 0 TD (of)Tj 12.767 0 TD (pect)Tj 22.002 0 TD (o)Tj 6.169 0 TD (lytic)Tj 24.234 0 TD (enzyme)Tj 39.328 0 TD (s)Tj 8.437 0 TD (that)Tj 23.264 0 TD (br)Tj 10.737 0 TD (e)Tj 5.844 0 TD (ak)Tj 15.011 0 TD (down the pectic)Tj 83.824 0 TD (s)Tj 5.687 0 TD (ub)Tj 12.925 0 TD (st)Tj 9.263 0 TD (anc)Tj 18.117 0 TD [ (e)21 (s)] TJ -524.47 -14.5 TD (of)Tj 12.517 0 TD (the middle lamel)Tj 85.755 0 TD (l)Tj 3.167 0 TD (a \(which cements)Tj 91.41 0 TD (c)Tj 5.185 0 TD (el)Tj 9.011 0 TD [ (l)-21 (s)-229 (t)] TJ 15.68 0 TD (ogether\), cau)Tj 67.129 0 TD (s)Tj 5.437 0 TD (ing br)Tj 29.769 0 TD (e)Tj 6.094 0 TD (ak)Tj 14.761 0 TD (down and maceration of)Tj 126.09 0 TD (the ti)Tj 26.337 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ues.)Tj -509.582 -14.5 TD (Slim)Tj 22.878 0 TD [ (y)-229 (ma)21 (s)] TJ 29.282 0 TD (se)Tj 11.78 0 TD (s)Tj 8.687 0 TD (of)Tj 12.767 0 TD (the bacteria and ac)Tj 97.566 0 TD (c)Tj 5.185 0 TD (ompan)Tj 35.3 0 TD (y)Tj 5.628 0 TD (ing cellu)Tj 42.898 0 TD (l)Tj 2.917 0 TD (ar debri)Tj 39.87 0 TD (s)Tj 8.437 0 TD (\322oo)Tj 18.404 0 TD [ (z)21 (e)] TJ 11.04 0 TD (s\323 out)Tj 34.22 0 TD (from c)Tj 31.536 0 TD (r)Tj 4.066 0 TD (ac)Tj 11.352 0 TD [ (k)21 (s)] TJ 14.53 0 TD (in the ti)Tj 39.25 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue and)Tj -498.592 -14.25 TD (become)Tj 39.763 0 TD [ (s)-250 (v)21 (i)21 (s)] TJ 22.545 0 TD (ib)Tj 9.85 0 TD (l)Tj 2.917 0 TD (e on the sur)Tj 61.054 0 TD (f)Tj 3.348 0 TD (ace. Ti)Tj 35.249 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue in the affected ar)Tj 102.232 0 TD (e)Tj 6.094 0 TD (a become)Tj 48.898 0 TD (s)Tj 8.687 0 TD (\322mush)Tj 34.002 0 TD (y)Tj 5.128 0 TD (,\323 c)Tj 17.161 0 TD (re)Tj 10.16 0 TD (am c)Tj 23.87 0 TD (o)Tj 6.419 0 TD (lored, and slim)Tj 76.459 0 TD [ (y)21 (.)] TJ -524.988 -14.5 TD (W)Tj 9.171 0 TD (ater diff)Tj 39.905 0 TD (u)Tj 6.504 0 TD (se)Tj 11.78 0 TD (s)Tj 8.437 0 TD (into the inter)Tj 66.171 0 TD (c)Tj 5.185 0 TD (ellu)Tj 18.682 0 TD (l)Tj 3.167 0 TD (ar s)Tj 18.638 0 TD (p)Tj 6.897 0 TD (ac)Tj 11.102 0 TD (es)Tj 14.53 0 TD (and cel)Tj 36.755 0 TD [ (l)21 (s)-250 (pl)21 (a)] TJ 27.335 0 TD 0.145 Tw (smolyze and die. The bacteria continue to mo)Tj 0 Tw 233.792 0 TD (ve)Tj -518.163 -14.25 TD (and multiply)Tj 66.729 0 TD (in the inter)Tj 55.925 0 TD (c)Tj 5.185 0 TD (ellu)Tj 18.682 0 TD (l)Tj 2.917 0 TD (ar s)Tj 18.888 0 TD (p)Tj 6.647 0 TD (ac)Tj 11.352 0 TD [ (e)21 (s)] TJ 14.53 0 TD (and cel)Tj 36.755 0 TD [ (l)21 (s)-229 (p)-21 (l)21 (a)] TJ 27.085 0 TD 0.176 Tw (smolyze and die. The bacteria continue to mo)Tj 0 Tw 234.042 0 TD (v)Tj 5.493 0 TD (e and)Tj -504.096 -14.5 TD (multiply)Tj 44.419 0 TD (in the inter)Tj 55.675 0 TD (c)Tj 5.185 0 TD (ellu)Tj 18.932 0 TD (l)Tj 2.917 0 TD (ar s)Tj 18.888 0 TD (p)Tj 6.397 0 TD (ac)Tj 11.602 0 TD [ (e)21 (s)] TJ 14.28 0 TD (and produce more enzyme)Tj 135.028 0 TD [ (s)-250 (f)] TJ 12.284 0 TD (or further ti)Tj 58.275 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue in)Tj 25.26 0 TD [ (va)21 (s)] TJ 16.847 0 TD (ion and)Tj -437.327 -14.5 TD (m)Tj 9.551 0 TD (aceration. The end r)Tj 104.052 0 TD (esu)Tj 18.284 0 TD (lt)Tj 9.743 0 TD [ (i)21 (s)] TJ 11.365 0 TD (that)Tj 23.264 0 TD (infected ti)Tj 51.372 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.437 0 TD (become sof)Tj 58.934 0 TD (t)Tj 6.576 0 TD (and a slim)Tj 53.028 0 TD [ (y)-229 (m)21 (as)] TJ 29.532 0 TD (s, of)Tj 22.114 0 TD (t)Tj 3.576 0 TD (en with a \322putrid\323)Tj -433.541 -14.25 TD 0.198 Tw (odor. An entire potato tuber or other )Tj 0 Tw 188.379 0 TD [ (v)21 (eg)] TJ 17.456 0 TD (etab)Tj 22.758 0 TD (l)Tj 2.917 0 TD (e m)Tj 18.613 0 TD [ (a)21 (y)] TJ 14.545 0 TD (become con)Tj 61.1 0 TD (v)Tj 5.493 0 TD (er)Tj 10.41 0 TD (t)Tj 3.576 0 TD (ed into thi)Tj 52.328 0 TD [ (s)-229 (w)] TJ 16.751 0 TD (ater)Tj 20.153 0 TD (y)Tj 5.378 0 TD (, dec)Tj 24.348 0 TD (ay)Tj 11.545 0 TD (ed m)Tj 25.272 0 TD [ (as)21 (s)] TJ -500.96 -14.5 TD (w)Tj 8.314 0 TD (ithin 3-5 d)Tj 51.457 0 TD (ay)Tj 11.795 0 TD (s! Because of)Tj 73.381 0 TD (the dramatic)Tj 67.382 0 TD (effects)Tj 37.33 0 TD (on ho)Tj 28.516 0 TD [ (s)-21 (t)] TJ 12.513 0 TD (ti)Tj 6.755 0 TD (ss)Tj 11.373 0 TD (ue and the rapidity)Tj 99.56 0 TD (of)Tj 13.017 0 TD (dise)Tj 21.118 0 TD (a)Tj 5.917 0 TD (se development,)Tj -448.5 -14.25 TD (sof)Tj 15.953 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD [ (i)21 (s)] TJ 11.865 0 TD (an e)Tj 21.494 0 TD [ (x)21 (c)] TJ 10.598 0 TD (el)Tj 9.261 0 TD (l)Tj 3.167 0 TD (ent)Tj 19.186 0 TD (model)Tj 34.64 0 TD (t)Tj 3.826 0 TD (o i)Tj 12.566 0 TD (l)Tj 2.917 0 TD (lu)Tj 9.671 0 TD (s)Tj 5.687 0 TD (trate p)Tj 33.345 0 TD (l)Tj 3.167 0 TD (ant)Tj 19.259 0 TD (dise)Tj 21.368 0 TD (a)Tj 5.917 0 TD (se development)Tj 84.058 0 TD (t)Tj 3.826 0 TD (o s)Tj 15.074 0 TD (t)Tj 3.576 0 TD (udents.)Tj /F2 12 Tf -373.831 -29 TD (Ru)Tj 13.871 0 TD [ (le)21 (s)-250 (o)21 (f)-271 (Pr)21 (o)21 (o)21 (f)-250 (o)21 (f)-292 (P)63 (a)63 (t)] TJ 102.658 0 TD (ho)Tj 14.267 0 TD (g)Tj 7.175 0 TD (enicit)Tj 30.905 0 TD (y)Tj /F3 12 Tf -169.065 -14.25 TD (The ru)Tj 31.881 0 TD (les)Tj 17.697 0 TD (of)Tj 12.767 0 TD (proof)Tj 29.899 0 TD (of)Tj 13.017 0 TD (p)Tj 6.647 0 TD (athogenicity)Tj 65.831 0 TD (w)Tj 8.314 0 TD (ere formu)Tj 49.11 0 TD (l)Tj 3.167 0 TD (ated in 1882 b)Tj 72.916 0 TD (y)Tj 8.628 0 TD (the German s)Tj 68.274 0 TD (c)Tj 5.185 0 TD (ienti)Tj 22.793 0 TD [ (s)21 (t)-250 (R)21 (o)] TJ 24.853 0 TD (ber)Tj 17.081 0 TD [ (t)-250 (K)] TJ 13.391 0 TD (och \(hence,)Tj -471.421 -14.5 TD (K)Tj 6.564 0 TD (och\325s)Tj 30.066 0 TD (po)Tj 12.816 0 TD [ (st)21 (ul)] TJ 18.934 0 TD (at)Tj 9.993 0 TD (e)Tj 5.844 0 TD (s\) from hi)Tj 48.68 0 TD [ (s)-229 (st)] TJ 17.699 0 TD (udie)Tj 22.436 0 TD (s)Tj 8.437 0 TD (on animal)Tj 53.649 0 TD (anthrax. These ru)Tj 91.331 0 TD [ (le)21 (s)-271 (w)] TJ 26.012 0 TD (ere a)Tj 25.389 0 TD (d)Tj 6.409 0 TD (apted to also determine the)Tj -384.107 -14.25 TD (actual)Tj 34.016 0 TD (c)Tj 5.185 0 TD (ause of)Tj 40.186 0 TD [ (p)21 (l)] TJ 9.564 0 TD (ant)Tj 19.509 0 TD (dise)Tj 21.118 0 TD (a)Tj 6.167 0 TD (se. Briefly)Tj 57.006 0 TD [ (s)21 (t)] TJ 9.263 0 TD (at)Tj 9.993 0 TD (e)Tj 5.844 0 TD (s, the ru)Tj 42.25 0 TD [ (le)21 (s)] TJ 17.447 0 TD (are a)Tj 25.712 0 TD [ (s)-229 (f)21 (ol)] TJ 21.12 0 TD (lo)Tj 9.836 0 TD (w)Tj 8.064 0 TD (s:)Tj -306.454 -14.5 TD (1. 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Place inoc)Tj 66.412 0 TD (ul)Tj 9.421 0 TD (ated )Tj 25.464 0 TD [ (v)-21 (e)21 (g)] TJ 17.956 0 TD (etab)Tj 22.508 0 TD (les)Tj 17.697 0 TD (onto a wet)Tj 56.267 0 TD (p)Tj 6.397 0 TD (aper t)Tj 29.769 0 TD (ow)Tj 14.733 0 TD (el)Tj 12.011 0 TD (in)Tj 9.444 0 TD (s)Tj 5.937 0 TD (ide a p)Tj 34.432 0 TD [ (l)21 (as)] TJ 14.771 0 TD (tic)Tj 15.189 0 TD [ (b)21 (a)-21 (g)] TJ -515.543 -14.5 TD (and seal)Tj 46.424 0 TD (tightly)Tj 35.439 0 TD (w)Tj 8.314 0 TD (ith a tw)Tj 37.693 0 TD [ (i)21 (st)] TJ 15.191 0 TD 0.22 Tw (tie. Use a separate b)Tj 0 Tw 107.674 0 TD (a)Tj 6.417 0 TD (g for eac)Tj 43.334 0 TD [ (h)-229 (v)-21 (e)21 (g)] TJ 27.4 0 TD (etab)Tj 22.508 0 TD (l)Tj 3.167 0 TD (e and separate b)Tj 85.509 0 TD [ (a)21 (gs)-229 (f)] TJ 24.57 0 TD (or the)Tj -463.682 -14.25 TD (c)Tj 5.435 0 TD (ontr)Tj 20.577 0 TD [ (o)-21 (l)21 (s)] TJ 18.022 0 TD (\(ver)Tj 19.192 0 TD (y)Tj 8.628 0 TD (impor)Tj 30.112 0 TD (t)Tj 3.576 0 TD (ant\).)Tj /F4 12 Tf -105.557 -29 TD [ (T)42 (o)-271 (T)21 (e)21 (s)42 (t)-229 (T)42 (em)] TJ 59.356 0 TD (pe)Tj 11.528 0 TD [ (r)42 (a)63 (tur)21 (e)-271 (Ef)42 (fe)] TJ 59.415 0 TD (ct)Tj 10.956 0 TD [ (s)-250 (o)] TJ 16.579 0 TD (n S)Tj 17.244 0 TD [ (o)21 (ft)] TJ 20.412 0 TD (Ro)Tj 13.93 0 TD (t)Tj /F3 12 Tf -209.436 -14.25 TD (Inoc)Tj 21.583 0 TD (ul)Tj 9.421 0 TD (ate the ti)Tj 45.642 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue slic)Tj 32.783 0 TD (es)Tj 14.53 0 TD (of)Tj 12.767 0 TD (who)Tj 21.428 0 TD [ (le)-229 (veg)] TJ 29.967 0 TD (etab)Tj 22.258 0 TD [ (le)21 (s)] TJ 17.947 0 TD (in a refrigerator, at)Tj 98.212 0 TD (r)Tj 4.066 0 TD (oom temperature, at)Tj 107.196 0 TD (26 degree)Tj 51.351 0 TD (s)Tj 8.687 0 TD (C,)Tj -508.72 -14.5 TD (and 45 degree)Tj 72.92 0 TD [ (s)-229 (C)] TJ 18.024 0 TD (if)Tj 9.776 0 TD (incubator)Tj 48.532 0 TD (s)Tj 8.437 0 TD (are avai)Tj 40.051 0 TD (l)Tj 2.917 0 TD (ab)Tj 12.838 0 TD (l)Tj 3.167 0 TD (e. Different)Tj 62.56 0 TD (group)Tj 29.756 0 TD (s)Tj 8.437 0 TD (of)Tj 12.767 0 TD (st)Tj 9.513 0 TD (udents)Tj 38.035 0 TD (c)Tj 5.185 0 TD (an choose one temperature.)Tj /F4 12 Tf -383.068 -28.75 TD (T)Tj 5.008 0 TD (o De)Tj 24.066 0 TD [ (te)21 (r)] TJ 16.81 0 TD (min)Tj 19.139 0 TD [ (e)-292 (E)21 (n)] TJ 21.99 0 TD (zy)Tj 10.416 0 TD [ (me)-271 (A)] TJ 24.461 0 TD (ctivit)Tj 29.417 0 TD [ (y)-250 (a)21 (n)] TJ 21.356 0 TD (d S)Tj 16.754 0 TD [ (o)-21 (f)21 (t)] TJ 20.912 0 TD (Ro)Tj 13.68 0 TD (t)Tj 8.314 0 TD (De)Tj 13.304 0 TD [ (ve)21 (l)42 (op)21 (m)] TJ 37.728 0 TD (en)Tj 12.526 0 TD (t)Tj /F3 12 Tf -295.936 -14.5 TD (The inoc)Tj 42.859 0 TD (ul)Tj 9.421 0 TD (ated ti)Tj 32.469 0 TD [ (s)-21 (s)] TJ 11.373 0 TD (ue slic)Tj 32.783 0 TD [ (e)21 (s)] TJ 14.53 0 TD (shou)Tj 25.054 0 TD (l)Tj 3.417 0 TD (d be chec)Tj 48.267 0 TD (k)Tj 5.844 0 TD (ed s)Tj 21.408 0 TD (t)Tj 3.576 0 TD (arting at)Tj 46.084 0 TD (48 hour)Tj 40.199 0 TD (s)Tj 8.437 0 TD (wher)Tj 25.169 0 TD [ (e)21 (as)] TJ 20.447 0 TD (the who)Tj 41.01 0 TD [ (le)-229 (veg)] TJ 29.717 0 TD (etab)Tj 22.508 0 TD (les)Tj -484.553 -14.25 TD (shou)Tj 25.304 0 TD (l)Tj 2.917 0 TD (d be chec)Tj 48.517 0 TD (k)Tj 5.844 0 TD (ed beginning at)Tj 83.089 0 TD (five d)Tj 27.741 0 TD [ (ay)21 (s)] TJ 20.231 0 TD (af)Tj 9.765 0 TD (t)Tj 3.576 0 TD (er inoc)Tj 34.926 0 TD [ (u)21 (l)] TJ 9.421 0 TD (ation. For the ti)Tj 80.173 0 TD [ (s)-21 (s)] TJ 11.373 0 TD (ue slic)Tj 32.783 0 TD (e)Tj 5.844 0 TD (s, t)Tj 16.173 0 TD [ (e)21 (s)21 (t)-250 (f)] TJ 21.704 0 TD (or ti)Tj 20.208 0 TD (ss)Tj 11.123 0 TD (ue)Tj -470.652 -14.5 TD (m)Tj 9.551 0 TD (aceration \(sof)Tj 69.828 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot\) b)Tj 23.185 0 TD (y)Tj 8.628 0 TD (running a transfer loop evenly)Tj 155.367 0 TD (ov)Tj 11.912 0 TD (er the slice and/or b)Tj 103.581 0 TD (y)Tj 8.378 0 TD (pr)Tj 10.714 0 TD (ob)Tj 13.09 0 TD (ing with a t)Tj 56.725 0 TD [ (e)21 (as)] TJ 17.447 0 TD (in)Tj 9.694 0 TD (g)Tj -508.631 -14.25 TD 0.198 Tw (needle. Do the same for the inoc)Tj 0 Tw 168.487 0 TD (ul)Tj 9.421 0 TD (ated ar)Tj 35.948 0 TD (e)Tj 6.094 0 TD (a on who)Tj 46.215 0 TD [ (le)-229 (veg)] TJ 29.717 0 TD (etab)Tj 22.508 0 TD (les)Tj 17.697 0 TD (\(be sure to use separate transfer loop)Tj 191.662 0 TD (s)Tj -527.813 -14.5 TD (or t)Tj 17.28 0 TD [ (e)21 (as)] TJ 17.447 0 TD (ing needl)Tj 47.561 0 TD [ (e)21 (s)-250 (f)] TJ 17.878 0 TD (or inoc)Tj 35.001 0 TD (ul)Tj 9.671 0 TD (ated ti)Tj 32.469 0 TD (ss)Tj 11.123 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.437 0 TD (and contr)Tj 48.572 0 TD [ (o)21 (l)] TJ 9.086 0 TD (s!\). Continue to e)Tj 89.842 0 TD (x)Tj 5.413 0 TD (amine for 2-3 d)Tj 77.256 0 TD [ (ay)21 (s)] TJ 19.981 0 TD (and note the)Tj -459.753 -14.5 TD (difference in rot)Tj 83.773 0 TD (produced on the different)Tj 132.815 0 TD (veg)Tj 17.706 0 TD (etab)Tj 22.508 0 TD [ (l)-21 (e)21 (s)] TJ 17.697 0 TD (and between the different)Tj 134.848 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.421 0 TD (ation)Tj -440.144 -14.25 TD (\(temperature\) conditions. Note the odor produced b)Tj 265.987 0 TD (y)Tj 8.378 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.421 0 TD (ated, rotting ti)Tj 73.081 0 TD (ss)Tj 11.373 0 TD (ue. Note: If)Tj 61.942 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.421 0 TD (ated)Tj -482.754 -14.5 TD (veg)Tj 17.706 0 TD (etab)Tj 22.508 0 TD [ (l)21 (es)] TJ 17.697 0 TD (in the b)Tj 38.916 0 TD (ags)Tj 20.973 0 TD (are incubated for appr)Tj 113.393 0 TD [ (o)21 (x)] TJ 11.832 0 TD (imately)Tj 40.111 0 TD (two week)Tj 47.873 0 TD (s)Tj 8.437 0 TD (at)Tj 12.993 0 TD (r)Tj 4.066 0 TD (oom temperature or 26 degree)Tj 155.815 0 TD [ (s)-229 (C)] TJ -512.226 -14.25 TD (they)Tj 24.992 0 TD [ (w)21 (i)-21 (l)21 (l)] TJ 20.327 0 TD (\322melt\323 and become almo)Tj 127.333 0 TD [ (st)-229 (t)] TJ 16.089 0 TD (otally)Tj 30.874 0 TD 0.27 Tw (liquefied due to the gr)Tj 0 Tw 113.544 0 TD (e)Tj 6.094 0 TD (at)Tj 12.743 0 TD (amount)Tj 41.732 0 TD (of)Tj 12.767 0 TD (pectic)Tj 33.365 0 TD (enzyme activity)Tj 78.529 0 TD (.)Tj /F4 12 Tf -518.366 -29 TD (T)Tj 4.758 0 TD (o De)Tj 24.066 0 TD [ (m)21 (ons)] TJ 28.525 0 TD (tr)Tj 11.22 0 TD [ (a)63 (t)] TJ 10.646 0 TD (e K)Tj 15.11 0 TD [ (o)21 (c)] TJ 13.104 0 TD (h\325s)Tj 19.386 0 TD [ (P)21 (os)42 (t)] TJ 24.72 0 TD (ul)Tj 12.259 0 TD [ (a)63 (t)21 (e)21 (s)] TJ /F3 12 Tf -163.896 -14.25 TD (Af)Tj 10.412 0 TD (t)Tj 3.826 0 TD (er s)Tj 18.815 0 TD (t)Tj 3.576 0 TD (udents)Tj 38.035 0 TD (h)Tj 6.694 0 TD (ave o)Tj 27.141 0 TD (b)Tj 6.421 0 TD (ser)Tj 16.097 0 TD (v)Tj 5.493 0 TD (ed the \322con)Tj 58.989 0 TD [ (s)21 (t)] TJ 9.263 0 TD (ant)Tj 19.259 0 TD (as)Tj 11.604 0 TD (soc)Tj 17.54 0 TD (i)Tj 3.179 0 TD (ation\323 between ti)Tj 87.972 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.437 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.421 0 TD (ated with Er)Tj 60.774 0 TD (w)Tj 8.314 0 TD (ini)Tj 12.623 0 TD (a)Tj -489.083 -14.5 TD (c)Tj 5.185 0 TD (arot)Tj 20.229 0 TD (ov)Tj 11.662 0 TD (ora and sof)Tj 57.633 0 TD [ (t)-250 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD (symptoms, have them iso)Tj 130.989 0 TD (l)Tj 3.167 0 TD (ate the bacterium \(u)Tj 102.641 0 TD (s)Tj 5.687 0 TD (ing a s)Tj 33.853 0 TD (t)Tj 3.826 0 TD (eri)Tj 13.089 0 TD (l)Tj 3.167 0 TD (e transfer loop\) int)Tj 94.491 0 TD (o)Tj -509.331 -14.25 TD (p)Tj 6.397 0 TD (ure c)Tj 25.067 0 TD (u)Tj 6.504 0 TD (lture on a nutrient)Tj 94.689 0 TD [ (a)-21 (g)] TJ 12.536 0 TD (ar p)Tj 19.849 0 TD (l)Tj 2.917 0 TD (ate \(compare with the pure c)Tj 145.469 0 TD (u)Tj 6.504 0 TD (lture used for the original)Tj 132.732 0 TD (inoc)Tj 21.548 0 TD [ (u)21 (l)] TJ 9.421 0 TD (ations\).)Tj -483.772 -14.5 TD (Af)Tj 10.662 0 TD (t)Tj 3.826 0 TD (er the pure c)Tj 64.175 0 TD (u)Tj 6.504 0 TD (lture h)Tj 33.07 0 TD [ (a)21 (s)] TJ 14.354 0 TD (gr)Tj 10.436 0 TD (o)Tj 6.419 0 TD (wn, use it)Tj 52.248 0 TD (t)Tj 3.576 0 TD (o inoc)Tj 31.185 0 TD [ (u)21 (l)] TJ 9.421 0 TD (at)Tj 9.743 0 TD [ (e)-250 (ve)21 (g)] TJ 26.8 0 TD (etab)Tj 22.508 0 TD [ (l)21 (e)-21 (s)-229 (b)21 (y)] TJ 32.746 0 TD (the procedure de)Tj 86.788 0 TD (sc)Tj 10.871 0 TD (ribed abo)Tj 48.894 0 TD (v)Tj 5.493 0 TD (e \(u)Tj 18.855 0 TD (s)Tj 5.437 0 TD (in)Tj 9.944 0 TD (g)Tj -523.881 -14.5 TD (the same )Tj 49.798 0 TD (veg)Tj 17.706 0 TD (etab)Tj 22.508 0 TD (l)Tj 2.917 0 TD (e sour)Tj 31.988 0 TD (c)Tj 5.185 0 TD (e\), o)Tj 22.224 0 TD (b)Tj 6.671 0 TD (ser)Tj 16.097 0 TD (v)Tj 5.493 0 TD (e the symptoms)Tj 84.139 0 TD (produced and then re-iso)Tj 128.689 0 TD (l)Tj 2.917 0 TD (ate the bacterium in pur)Tj 122.728 0 TD (e)Tj -518.906 -14.25 TD [ (c)21 (u)] TJ 11.688 0 TD (lture \(compare with original)Tj 143.805 0 TD [ (c)21 (u)] TJ 11.688 0 TD (lture used to inoc)Tj 89.048 0 TD (ul)Tj 9.421 0 TD (ate these )Tj 50.168 0 TD [ (v)21 (eg)] TJ 17.706 0 TD (etab)Tj 22.258 0 TD [ (l)-21 (e)] TJ 9.261 0 TD (s.)Tj /F2 12 Tf -365.18 -28.75 TD (Exten)Tj 29.287 0 TD (s)Tj 6.004 0 TD (io)Tj 10.883 0 TD (n)Tj /F3 12 Tf -46.219 -14.5 TD (Have s)Tj 33.909 0 TD (t)Tj 3.576 0 TD (udents)Tj 38.035 0 TD [ (r)-21 (e)] TJ 10.41 0 TD (sear)Tj 21.764 0 TD (c)Tj 5.185 0 TD (h Koch\325s)Tj 46.043 0 TD (w)Tj 8.064 0 TD (ork)Tj 19.329 0 TD (w)Tj 8.064 0 TD (ith anthrax)Tj 58.517 0 TD (and di)Tj 31.898 0 TD [ (scus)21 (s)] TJ 31.498 0 TD (the simi)Tj 40.927 0 TD (l)Tj 3.167 0 TD (aritie)Tj 26.511 0 TD (s)Tj 8.437 0 TD (between hi)Tj 56.2 0 TD (s)Tj 8.437 0 TD (findin)Tj 29.896 0 TD [ (g)21 (s)] TJ 14.806 0 TD (and)Tj -504.658 -14.5 TD (the procedur)Tj 64.973 0 TD [ (e)-21 (s)] TJ 14.78 0 TD (from these activities.)Tj -79.588 -28.75 TD (Ap)Tj 13.462 0 TD (p)Tj 6.397 0 TD (ly)Tj 11.795 0 TD (c)Tj 5.185 0 TD (ommer)Tj 35.681 0 TD [ (c)21 (i)] TJ 8.363 0 TD (ally)Tj 20.879 0 TD (p)Tj 6.647 0 TD (ur)Tj 10.57 0 TD [ (c)42 (h)21 (a)] TJ 17.796 0 TD (sed pectin)Tj 52.604 0 TD (a)Tj 6.167 0 TD (se t)Tj 18.575 0 TD [ (o)-229 (veg)] TJ 27.125 0 TD (etab)Tj 22.508 0 TD (l)Tj 2.917 0 TD (e samp)Tj 36.864 0 TD [ (le)21 (s)] TJ 17.697 0 TD (and compare the r)Tj 92.806 0 TD (esu)Tj 18.034 0 TD (lts)Tj 15.93 0 TD (w)Tj 7.814 0 TD (ith what)Tj -455.831 -14.25 TD (h)Tj 6.444 0 TD (appened with the bacteria.)Tj /F2 12 Tf -6.405 -29 TD [ (S)63 (t)21 (ud)] TJ 25.563 0 TD (en)Tj 12.431 0 TD (t)Tj 8.111 0 TD (Qu)Tj 15.548 0 TD [ (e)21 (s)] TJ 11.403 0 TD (tio)Tj 16.244 0 TD [ (n)21 (s)] TJ 16.285 0 TD (\(a)Tj 9.77 0 TD (n)Tj 7.031 0 TD (d a)Tj 15.949 0 TD [ (n)21 (s)21 (we)] TJ 26.916 0 TD (rs)Tj 12.326 0 TD (\))Tj /F3 12 Tf -177.73 -14.25 TD (1. Wh)Tj 27.53 0 TD (y)Tj 8.628 0 TD (are pectic)Tj 52.66 0 TD (polymer)Tj 41.572 0 TD (s)Tj 8.437 0 TD (impor)Tj 29.862 0 TD (t)Tj 3.826 0 TD (ant)Tj 19.259 0 TD (in p)Tj 19.31 0 TD (l)Tj 3.167 0 TD (ants)Tj 24.945 0 TD (and what)Tj 50.062 0 TD [ (i)21 (s)] TJ 11.615 0 TD (their f)Tj 30.425 0 TD (u)Tj 6.254 0 TD (nction? Pectic)Tj 73.594 0 TD (polymer)Tj 41.072 0 TD (s)Tj 8.937 0 TD (ar)Tj 9.983 0 TD (e)Tj -471.156 -14.5 TD (c)Tj 5.185 0 TD (ompound)Tj 48.715 0 TD (s)Tj 8.437 0 TD (that)Tj 23.264 0 TD (help pr)Tj 36.284 0 TD [ (o)21 (v)] TJ 11.662 0 TD (ide suppor)Tj 55.12 0 TD [ (t)-208 (f)] TJ 9.924 0 TD (or cel)Tj 27.899 0 TD (l)Tj 3.167 0 TD (s. Pectin)Tj 46.083 0 TD (s)Tj 8.687 0 TD (pr)Tj 10.714 0 TD [ (o)21 (v)] TJ 11.662 0 TD (ide stren)Tj 44.838 0 TD (g)Tj 6.369 0 TD (th b)Tj 20.159 0 TD (y)Tj 8.378 0 TD (helping to bind \(cement\))Tj -386.556 -14.25 TD (c)Tj 5.435 0 TD (el)Tj 8.761 0 TD [ (l)-21 (s)-229 (t)] TJ 15.68 0 TD (ogether into ti)Tj 72.193 0 TD [ (s)21 (s)] TJ 10.873 0 TD (ues.)Tj -112.832 -14.5 TD (2. How doe)Tj 56.991 0 TD (s)Tj 8.687 0 TD (Er)Tj 10.078 0 TD (w)Tj 8.314 0 TD (inia carot)Tj 47.421 0 TD (ov)Tj 11.662 0 TD (ora utilize the \322dig)Tj 94.839 0 TD (est)Tj 15.356 0 TD (ed\323 p)Tj 28.185 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.259 0 TD (ti)Tj 7.005 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue a)Tj 21.733 0 TD (s)Tj 8.437 0 TD (a food sour)Tj 57.624 0 TD (c)Tj 5.435 0 TD (e? Plant)Tj 43.256 0 TD (p)Tj 6.397 0 TD (athogenic)Tj -464.802 -14.5 TD (b)Tj 6.421 0 TD (acteria, such a)Tj 74.298 0 TD (s)Tj 8.687 0 TD (Er)Tj 10.078 0 TD (w)Tj 8.314 0 TD (inia carot)Tj 47.421 0 TD (ov)Tj 11.912 0 TD (ora sec)Tj 36.335 0 TD (r)Tj 4.066 0 TD (ete enzyme)Tj 58.31 0 TD [ (s)-229 (s)] TJ 14.123 0 TD (uch a)Tj 27.268 0 TD (s)Tj 8.437 0 TD (pectin)Tj 31.696 0 TD (a)Tj 6.167 0 TD (se)Tj 11.53 0 TD (s)Tj 8.687 0 TD (that)Tj 23.264 0 TD (degr)Tj 22.938 0 TD (a)Tj 6.167 0 TD (de p)Tj 22.118 0 TD (l)Tj 3.167 0 TD (ant)Tj 19.259 0 TD (c)Tj 5.435 0 TD (el)Tj 9.011 0 TD (ls)Tj 11.604 0 TD (and)Tj ET endstream endobj 24 0 obj 15525 endobj 22 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F2 9 0 R /F3 11 0 R /F4 15 0 R >> /ProcSet 2 0 R >> /Contents 23 0 R >> endobj 26 0 obj << /Length 27 0 R >> stream 0.25 w 1 g 36.25 36.75 539 719 re f BT /F3 12 Tf 0 g 37.394 745.25 TD 0 Tc 0 Tw (ti)Tj 7.005 0 TD [ (s)21 (s)] TJ 10.873 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.687 0 TD (and then utilize the products)Tj 150.096 0 TD (of)Tj 13.017 0 TD (dig)Tj 15.957 0 TD (es)Tj 11.78 0 TD (tion b)Tj 29.328 0 TD (y)Tj 8.378 0 TD (absorbing them.)Tj -267.739 -14.25 TD (3. Do other p)Tj 66.083 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.509 0 TD (p)Tj 6.397 0 TD (athogen)Tj 41.835 0 TD [ (s)-250 (s)] TJ 14.373 0 TD (uch a)Tj 27.018 0 TD [ (s)-271 (f)21 (u)21 (n)] TJ 25.054 0 TD (gi and )Tj 34.826 0 TD (v)Tj 5.243 0 TD (iruse)Tj 25.529 0 TD (s)Tj 8.437 0 TD (produce cel)Tj 58.738 0 TD [ (l)-250 (w)] TJ 14.231 0 TD (all-degr)Tj 39.075 0 TD (a)Tj 6.167 0 TD (ding enzymes? In a)Tj 97.529 0 TD (d)Tj 6.659 0 TD (dition)Tj -499.723 -14.5 TD (t)Tj 3.826 0 TD (o p)Tj 16.034 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.509 0 TD (p)Tj 6.647 0 TD (athogenic)Tj 53.448 0 TD (b)Tj 6.421 0 TD (acteria, f)Tj 44.692 0 TD (un)Tj 12.77 0 TD (gi also produce enzyme)Tj 120.794 0 TD (s)Tj 8.437 0 TD (that)Tj 23.264 0 TD (are sec)Tj 36.26 0 TD (r)Tj 4.066 0 TD (eted into infected p)Tj 99.313 0 TD (l)Tj 3.167 0 TD (ant)Tj 19.259 0 TD (ti)Tj 7.005 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue)Tj 12.348 0 TD (s)Tj -511.313 -14.5 TD (and the products)Tj 90.135 0 TD (of)Tj 12.767 0 TD (enzymatic)Tj 54.341 0 TD (activity)Tj 39.732 0 TD (pr)Tj 10.714 0 TD [ (o)21 (v)] TJ 11.412 0 TD (ide nutrients)Tj 68.113 0 TD (f)Tj 3.348 0 TD (or the f)Tj 36.883 0 TD [ (u)21 (n)] TJ 12.77 0 TD (gus. )Tj 27.38 0 TD (V)Tj 6.814 0 TD (iruse)Tj 25.279 0 TD (s)Tj 8.687 0 TD (that)Tj 23.264 0 TD (infect)Tj 31.146 0 TD [ (p)-21 (l)] TJ 9.814 0 TD (ants)Tj 24.945 0 TD (do not)Tj -497.443 -14.25 TD (produce enzymes.)Tj -0.059 -14.5 TD (4. What)Tj 42.096 0 TD [ (wa)21 (s)] TJ 22.668 0 TD (the effect)Tj 50.976 0 TD (of)Tj 12.767 0 TD (t)Tj 3.826 0 TD (emperature on the rate of)Tj 133.2 0 TD (the sof)Tj 35.285 0 TD [ (t)-250 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD (e)Tj 6.094 0 TD (xperiment? Why? Refrigeration th)Tj 169.279 0 TD (w)Tj 8.314 0 TD (arts)Tj -508.004 -14.25 TD (r)Tj 3.816 0 TD (ot)Tj 12.995 0 TD (development)Tj 69.56 0 TD (as)Tj 14.604 0 TD (doe)Tj 19.172 0 TD [ (s)-229 (e)] TJ 14.53 0 TD (xtremely)Tj 46.839 0 TD (high temperatur)Tj 82.496 0 TD [ (e)21 (s)] TJ 14.53 0 TD (because it)Tj 55.123 0 TD (arr)Tj 14.3 0 TD (es)Tj 11.78 0 TD (ts)Tj 12.263 0 TD (gr)Tj 10.186 0 TD (o)Tj 6.419 0 TD (wth of)Tj 34.82 0 TD (Er)Tj 10.078 0 TD (w)Tj 8.064 0 TD (inia carot)Tj 47.671 0 TD (ov)Tj 11.662 0 TD (or)Tj 10.235 0 TD (a)Tj -511.083 -14.5 TD (and minimiz)Tj 62.659 0 TD [ (e)21 (s)] TJ 14.53 0 TD 0.163 Tw (enzyme production. Room temperature and 26 degree)Tj 0 Tw 278.378 0 TD [ (s)-229 (C)] TJ 18.024 0 TD (are more conducive to bacterial)Tj -373.672 -14.5 TD (gr)Tj 10.186 0 TD (o)Tj 6.419 0 TD 0.27 Tw (wth and enzyme production. A)Tj 0 Tw 158.213 0 TD [ (l)21 (l)-229 (p)-21 (l)] TJ 18.648 0 TD (ant)Tj 19.259 0 TD (p)Tj 6.647 0 TD (athogenic)Tj 53.448 0 TD (b)Tj 6.671 0 TD (acteria have an optimum temperature for gr)Tj 222.254 0 TD (o)Tj 6.169 0 TD (wth)Tj -507.915 -14.25 TD (and pathogene)Tj 76.886 0 TD (si)Tj 8.615 0 TD (s.)Tj -85.43 -14.5 TD (5. What)Tj 41.09 0 TD (do the p)Tj 42.276 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.259 0 TD (m)Tj 9.551 0 TD (aterial)Tj 32.416 0 TD (s)Tj 8.687 0 TD (\(v)Tj 9.032 0 TD [ (e)21 (g)] TJ 12.213 0 TD (etab)Tj 22.508 0 TD (le)Tj 9.261 0 TD (s\) used in thi)Tj 66.228 0 TD [ (s)-229 (e)] TJ 14.53 0 TD (xperiment)Tj 54.386 0 TD (al)Tj 9.334 0 TD [ (l)-229 (h)] TJ 12.361 0 TD (ave in common? A)Tj 92.048 0 TD [ (l)21 (l)] TJ 9.084 0 TD (of)Tj 13.017 0 TD (these)Tj -480.355 -14.25 TD (veg)Tj 17.706 0 TD (etab)Tj 22.508 0 TD [ (le)21 (s)-271 (c)] TJ 23.132 0 TD (on)Tj 12.685 0 TD [ (s)21 (ist)] TJ 21.128 0 TD (of)Tj 12.517 0 TD (fl)Tj 6.765 0 TD (e)Tj 6.094 0 TD (sh)Tj 12.381 0 TD [ (y)-229 (st)] TJ 17.891 0 TD (or)Tj 10.235 0 TD (ag)Tj 12.536 0 TD (e or)Tj 19.547 0 TD (g)Tj 6.119 0 TD (an)Tj 12.433 0 TD (s)Tj 8.687 0 TD (that)Tj 23.264 0 TD (are soft, contain ab)Tj 98.598 0 TD (u)Tj 6.254 0 TD (ndant)Tj 32.684 0 TD (moi)Tj 18.898 0 TD (st)Tj 9.513 0 TD (ure, and are high in)Tj -411.471 -14.5 TD (c)Tj 5.185 0 TD (arboh)Tj 30.018 0 TD (y)Tj 5.378 0 TD (drat)Tj 20.719 0 TD (e)Tj 5.844 0 TD (s.)Tj -67.18 -14.5 TD [ (6)21 (.)] TJ 12.625 0 TD (Wh)Tj 16.615 0 TD (y)Tj 8.378 0 TD (did the sliced ti)Tj 78.74 0 TD (ss)Tj 11.373 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.437 0 TD (develop sof)Tj 59.244 0 TD [ (t)-250 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD [ (fa)21 (st)] TJ 18.777 0 TD (er than the who)Tj 80.31 0 TD [ (l)21 (e)-250 (veg)] TJ 29.717 0 TD (etab)Tj 22.508 0 TD (l)Tj 2.917 0 TD (e? The slic)Tj 52.831 0 TD [ (es)-229 (w)] TJ 22.845 0 TD (ere alr)Tj 32.372 0 TD (ea)Tj 12.011 0 TD (dy)Tj -505.963 -14.25 TD (pre-wounded b)Tj 76.997 0 TD [ (y)-229 (c)] TJ 13.813 0 TD (utting prior to inoc)Tj 95.045 0 TD (ul)Tj 9.421 0 TD 0.198 Tw (ation and there were more bacteria per unit)Tj 224.384 0 TD 0 Tw (of)Tj 13.017 0 TD (ti)Tj 6.755 0 TD (ss)Tj 11.373 0 TD (ue a)Tj 21.483 0 TD [ (s)-229 (c)] TJ 13.621 0 TD (ompared)Tj -485.896 -14.5 TD (w)Tj 8.314 0 TD (ith the who)Tj 57.427 0 TD [ (le)-229 (veg)] TJ 29.717 0 TD (etab)Tj 22.508 0 TD (l)Tj 3.167 0 TD (e.)Tj -121.022 -14.25 TD [ (7)125 (.)] TJ 10.655 0 TD (What)Tj 29.108 0 TD (is)Tj 11.615 0 TD (the impor)Tj 49.445 0 TD (t)Tj 3.826 0 TD (ance of)Tj 39.696 0 TD (K)Tj 6.814 0 TD (och\325s)Tj 29.816 0 TD (po)Tj 12.816 0 TD [ (s)-21 (t)21 (u)21 (l)] TJ 19.184 0 TD (at)Tj 9.743 0 TD (e)Tj 5.844 0 TD (s? Koch\325s)Tj 50.527 0 TD (po)Tj 13.066 0 TD [ (s)21 (tul)] TJ 18.684 0 TD (at)Tj 9.993 0 TD (es)Tj 14.53 0 TD (are utilized to pr)Tj 83.744 0 TD [ (o)21 (v)] TJ 11.662 0 TD (e the actual)Tj 62.91 0 TD (c)Tj 5.185 0 TD (ause of)Tj -499.064 -14.5 TD (a given p)Tj 46.401 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.509 0 TD (dise)Tj 21.368 0 TD (a)Tj 5.917 0 TD (se. )Tj 20.85 0 TD (W)Tj 9.921 0 TD (ithout)Tj 33.198 0 TD (kno)Tj 19.028 0 TD (w)Tj 8.064 0 TD (ing the cause it)Tj 80.973 0 TD (is)Tj 11.615 0 TD (impo)Tj 25.796 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ib)Tj 9.6 0 TD (l)Tj 3.417 0 TD (e to develop effective contr)Tj 137.705 0 TD (ol)Tj 12.336 0 TD (me)Tj 15.645 0 TD [ (a)21 (s)] TJ 11.604 0 TD (ur)Tj 10.57 0 TD (es)Tj -517.47 -14.5 TD (f)Tj 3.598 0 TD (or an)Tj 25.886 0 TD (y)Tj 8.628 0 TD (dise)Tj 21.368 0 TD (a)Tj 5.917 0 TD (se.)Tj /F5 10 Tf -65.336 -27 TD (T)Tj 3.883 0 TD (aken fr)Tj 33.55 0 TD (o)Tj 6.054 0 TD (m Plan)Tj 31.28 0 TD [ (t)-250 (P)75 (a)50 (t)25 (h)] TJ 26.804 0 TD (ol)Tj 10.487 0 TD [ (o)25 (g)25 (y)-225 (T)] TJ 22.772 0 TD (ea)Tj 10.226 0 TD (c)Tj 5.003 0 TD (her )Tj 18.413 0 TD (W)Tj 7.615 0 TD (ork)Tj 16.741 0 TD (sh)Tj 11.457 0 TD (op, 2001, Uni)Tj 57.67 0 TD (v)Tj 4.773 0 TD (ersi)Tj 18.06 0 TD [ (ty)-250 (o)] TJ 18.108 0 TD (f Georgia Depar)Tj 72.423 0 TD (t)Tj 4.461 0 TD (men)Tj 17.882 0 TD [ (t)-250 (o)] TJ 13.265 0 TD (f Plan)Tj 27.737 0 TD [ (t)-250 (P)50 (a)50 (t)25 (h)] TJ 27.054 0 TD (ol)Tj 10.237 0 TD [ (og)25 (y)125 (.)] TJ -476.038 -13.75 TD (Prepared by: Rober)Tj 87.623 0 TD (t)Tj 6.961 0 TD (B. Carroll)Tj 50.09 0 TD (and )Tj 19.872 0 TD [ (T)75 (h)-25 (o)50 (m)-25 (a)] TJ 28.618 0 TD (s A. E)Tj 24.366 0 TD (v)Tj 5.023 0 TD (ans, Depar)Tj 48.427 0 TD (t)Tj 4.211 0 TD (men)Tj 17.882 0 TD [ (t)-250 (o)] TJ 13.265 0 TD (f Plan)Tj 27.737 0 TD (t)Tj 6.961 0 TD (and Soil)Tj 41.503 0 TD (S)Tj 6.023 0 TD (cien)Tj 18.811 0 TD [ (c)25 (e)] TJ 9.707 0 TD (s, Uni)Tj 26.163 0 TD (v)Tj 4.773 0 TD (ersi)Tj 18.31 0 TD [ (ty)-250 (o)] TJ 17.858 0 TD (f Del)Tj 22.434 0 TD (aw)Tj 12.008 0 TD (are)Tj ET q 163.25 0 0 53.75 409 37.25 cm /I1 Do endstream endobj 27 0 obj 7633 endobj 30 0 obj << /Type /XObject /Subtype /Image /Name /I1 /Filter /DCTDecode /Width 682 /Height 225 /BitsPerComponent 8 /ColorSpace /DeviceRGB /Length 31 0 R >> stream AdobedC+ "+6.++(:SRB3BMPef`bb`knuruvh}  a!1AQa"q2B#$Rb34rC%Scs5&DTdE£t6UeuF'Vfv7GWgw(8HXhx )9IYiy *:JZjz ?F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$HIHwSy>úO0;5!޲±ⵁκ.ϱ՗:ðA+ <WiXS aWiXS aWiXS a*EAAv(Ue,"#Gʫ.edĐAAF4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F5Oٙm5`y§l0FSf`[Mn#^`,ïhkpէ Y^9cwO} L+r視Vj_Nَ&}:f;HFꟽ"a=ZoKp68zޖmݷq+k-ۻoxU&GY{a '&GY{a '&GY{a 'qۺUqۺUqۺP?kid~黃~&9@wLr_ela{'q:.Vw_ela{'q:(ci0 ;L†>N>H&[`3$F4I$_F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$ӹ21Q;qvW2qW[=:'{9.~JN8pǺUDg!Oyس^U.e6w|Gc"/;kʥן̲VebyTPC/c͹{@mM vC/c͹{@mM vC/c͹{@mM v[N^m[s7;EF_U/6ڭӝ/*ӗmVQ/{~%hy=I?K<㇞y$SoJqkkt8|ҺyZ>Zs WȤi5deau-9 ȃVFVYܓ[>immPeS PeS PeS ַl}o4XdN[p>Ʒ,2'Py8~[c[yZXUek:A,Lm V&UYt6·yi($F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I"@xH?nOQ~}GY{'s-9o61wc@0\.e=G5mf5 h B+|۔?Z[ܦX<qШu 62+W }m¶_aEeAʃyͿg}?%[eonWe`y~K62ݞ;`:>95)贱)ɬ۰ڵѲ^/H. q=|=LH>*q==L>*q==L>*LηɯU>0ձņT|ʡAT cj#ϼ2P©k{{OF_soU9p/ob;pmC 7G}?C]m89&jps\']>Mw1O p p pܨPpcw[* `n\c+rEA Kw%G':j]mkOQΧ[irsƥv}GP6ak$N>CGP6ak$N>CGP6ak$N>CܜfXgp5ܜfXgp5ܜfXgp5ѹgR%2 ewbd2ԪoYhث u*[ Z;v*>'Q9k*lqUKǵ6 ]Q󪥏zc ylL7ʚK Ēpf&eM%\I[8}3 .q$jڶF4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$W]cكvp%w?Z}J{0q.x O]uf9e?O)m42|J54Sil>djh |*3%Fd̔JȔJȔJȕշ*슞U@x{WdTLzămʻ":c$%VmٖqiZzͷfZ!Ŧ$xwUiO6ݙkpZt>RlZt>RlZt>RlYocxlh1V7ƁYocxlhվ]P~;zB]P~;zB]P~;zBI8 ,nM7JI8 ,nM7JI8 ,nM7Jg_iN U :KH u2YZ@mu0|*ye@XxWnO[,urgh rzYc+;@0>+szǧjۀii$N;S>II'wYnݧ|Vѝcq\)npuwgdXW n;]nm7›.lAn&w0G=V×W-dHrrLZ{I+*ךKƺ!}BҭyԽkW{O+*ךKƺ!}BF4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4Ieu_^ii}.gdrdm~}yuVWQ斗zVG-AFT.#h~iz57?"qCkOfѩ Z{7޿ZY9mדG'8vwh$^N;䭵@ֆ Z4 YI\ccu# YI\ccu# YI\ccu#/F4#H{FLJ5^<8@fwP'oNqӱЫȗ8R0XUK)y忘Ȭgt*%?AU6:8yQmh,3AU6:8ySH-h M %66v[u4ؖoRƳ5knkL-f,k3_vpoRƳ5knkL!Ǵ_ 3落c;z}!} xboO~0ϾaT.*I_Wu`SuoM?:  ëi"Q԰V!ǀ[? 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Ti)Tj 35.249 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue in the affected ar)Tj 102.232 0 TD (e)Tj 6.094 0 TD (a become)Tj 48.898 0 TD (s)Tj 8.437 0 TD (\322mush)Tj 34.002 0 TD (y)Tj 5.378 0 TD (,\323 c)Tj 17.161 0 TD (re)Tj 10.16 0 TD (am c)Tj 23.87 0 TD (o)Tj 6.419 0 TD (lored, and slim)Tj 76.459 0 TD [ (y)21 (.)] TJ -524.988 -14.5 TD (W)Tj 9.171 0 TD (ater diff)Tj 39.905 0 TD (u)Tj 6.504 0 TD (se)Tj 11.78 0 TD (s)Tj 8.437 0 TD (into the inter)Tj 66.171 0 TD (c)Tj 5.185 0 TD (ellu)Tj 18.682 0 TD (l)Tj 3.167 0 TD (ar s)Tj 18.638 0 TD (p)Tj 6.897 0 TD (ac)Tj 11.102 0 TD (es)Tj 14.53 0 TD (and cel)Tj 36.755 0 TD [ (l)21 (s)-250 (pl)21 (a)] TJ 27.335 0 TD 0.145 Tw (smolyze and die. The bacteria continue to mo)Tj 0 Tw 233.792 0 TD (ve)Tj -518.163 -14.25 TD (and multiply)Tj 66.729 0 TD (in the inter)Tj 55.675 0 TD (c)Tj 5.435 0 TD (ellu)Tj 18.682 0 TD (l)Tj 2.917 0 TD (ar s)Tj 18.888 0 TD (p)Tj 6.647 0 TD (ac)Tj 11.352 0 TD [ (e)21 (s)] TJ 14.53 0 TD (and cel)Tj 36.755 0 TD [ (l)21 (s)-229 (p)-21 (l)21 (a)] TJ 27.085 0 TD 0.176 Tw (smolyze and die. The bacteria continue to mo)Tj 0 Tw 234.042 0 TD (v)Tj 5.493 0 TD (e and)Tj -504.096 -14.5 TD (multiply)Tj 44.419 0 TD (in the inter)Tj 55.675 0 TD (c)Tj 5.185 0 TD (ellu)Tj 18.932 0 TD (l)Tj 2.917 0 TD (ar s)Tj 18.888 0 TD (p)Tj 6.397 0 TD (ac)Tj 11.602 0 TD [ (e)21 (s)] TJ 14.28 0 TD (and produce more enzyme)Tj 135.028 0 TD [ (s)-250 (f)] TJ 12.284 0 TD (or further ti)Tj 58.275 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue in)Tj 25.26 0 TD [ (va)21 (s)] TJ 16.847 0 TD (ion and)Tj -437.327 -14.5 TD (m)Tj 9.551 0 TD (aceration. The end r)Tj 104.052 0 TD (esu)Tj 18.284 0 TD (lt)Tj 9.743 0 TD [ (i)21 (s)] TJ 11.365 0 TD (that)Tj 23.264 0 TD (infected ti)Tj 51.372 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.437 0 TD (become sof)Tj 58.934 0 TD (t)Tj 6.576 0 TD (and a slim)Tj 53.028 0 TD [ (y)-229 (m)21 (as)] TJ 29.532 0 TD (s, of)Tj 22.114 0 TD (t)Tj 3.576 0 TD (en with a \322putrid\323)Tj -433.541 -14.25 TD 0.198 Tw (odor. An entire potato tuber or other )Tj 0 Tw 188.379 0 TD [ (v)21 (eg)] TJ 17.456 0 TD (etab)Tj 22.758 0 TD (l)Tj 2.917 0 TD (e m)Tj 18.613 0 TD [ (a)21 (y)] TJ 14.545 0 TD (become con)Tj 61.1 0 TD (v)Tj 5.493 0 TD (er)Tj 10.41 0 TD (t)Tj 3.576 0 TD (ed into thi)Tj 52.328 0 TD [ (s)-229 (w)] TJ 16.751 0 TD (ater)Tj 20.153 0 TD (y)Tj 5.378 0 TD (, dec)Tj 24.348 0 TD (ay)Tj 11.545 0 TD (ed m)Tj 25.272 0 TD [ (as)21 (s)] TJ -500.96 -14.5 TD (w)Tj 8.314 0 TD (ithin 3-5 d)Tj 51.457 0 TD (ay)Tj 11.795 0 TD (s! Because of)Tj 73.381 0 TD (the dramatic)Tj 67.382 0 TD (effects)Tj 37.33 0 TD (on ho)Tj 28.516 0 TD [ (s)-21 (t)] TJ 12.513 0 TD (ti)Tj 6.755 0 TD (ss)Tj 11.373 0 TD (ue and the rapidity)Tj 99.56 0 TD (of)Tj 13.017 0 TD (dise)Tj 21.118 0 TD (a)Tj 5.917 0 TD (se development,)Tj -448.5 -14.25 TD (sof)Tj 15.953 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD [ (i)21 (s)] TJ 11.865 0 TD (an e)Tj 21.494 0 TD [ (x)21 (c)] TJ 10.598 0 TD (el)Tj 9.261 0 TD (l)Tj 3.167 0 TD (ent)Tj 19.186 0 TD (model)Tj 34.64 0 TD (t)Tj 3.826 0 TD (o i)Tj 12.566 0 TD (l)Tj 2.917 0 TD (lu)Tj 9.671 0 TD (s)Tj 5.687 0 TD (trate p)Tj 33.345 0 TD (l)Tj 3.167 0 TD (ant)Tj 19.009 0 TD (dise)Tj 21.618 0 TD (a)Tj 5.917 0 TD (se development)Tj 84.058 0 TD (t)Tj 3.826 0 TD (o s)Tj 15.074 0 TD (t)Tj 3.576 0 TD (udents.)Tj /F2 12 Tf -373.831 -29 TD [ (P)21 (r)21 (o)21 (ced)42 (ur)21 (e)] TJ /F3 12 Tf -0.044 -14.25 TD [ (A)-229 (v)] TJ 14.808 0 TD (ariety)Tj 31.96 0 TD (of)Tj 13.017 0 TD (e)Tj 5.844 0 TD (xperimental)Tj 63.72 0 TD (c)Tj 5.185 0 TD (hoic)Tj 21.477 0 TD [ (e)21 (s)-250 (exi)21 (st)] TJ 41.479 0 TD (in in)Tj 22.107 0 TD (ves)Tj 17.273 0 TD (tigating bacterial)Tj 89.67 0 TD (sof)Tj 15.703 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot. B)Tj 23.772 0 TD (e)Tj 6.094 0 TD (gin b)Tj 25.452 0 TD (y)Tj 8.378 0 TD (preparing fr)Tj 60.384 0 TD (e)Tj 5.844 0 TD (sh)Tj -482.869 -14.5 TD (nutrient)Tj 43.276 0 TD (ag)Tj 12.536 0 TD (ar t)Tj 17.028 0 TD [ (e)21 (st)-229 (t)] TJ 21.933 0 TD (ube s)Tj 27.673 0 TD (l)Tj 3.417 0 TD (ants)Tj 24.945 0 TD (on Petri p)Tj 48.961 0 TD (l)Tj 2.917 0 TD (ate c)Tj 24.49 0 TD (u)Tj 6.254 0 TD (ltur)Tj 17.563 0 TD (es)Tj 14.53 0 TD (of)Tj 13.017 0 TD (Er)Tj 9.828 0 TD (w)Tj 8.314 0 TD (inia carot)Tj 47.671 0 TD (ov)Tj 11.662 0 TD (ora. A helpf)Tj 58.737 0 TD (ul)Tj 12.421 0 TD (guide for thi)Tj 62.523 0 TD [ (s)-229 (is)] TJ 20.052 0 TD (the)Tj -509.636 -14.25 TD (p)Tj 6.397 0 TD (ublication from NABT referenced in the w)Tj 207.682 0 TD (e)Tj 6.094 0 TD (b r)Tj 13.455 0 TD (e)Tj 6.344 0 TD (sour)Tj 22.426 0 TD [ (c)21 (es)] TJ 19.715 0 TD (abo)Tj 19.257 0 TD (v)Tj 5.243 0 TD (e. Some po)Tj 57.275 0 TD (ss)Tj 11.123 0 TD (ib)Tj 9.85 0 TD (l)Tj 2.917 0 TD (e activities/in)Tj 70.196 0 TD [ (ve)21 (s)] TJ 17.023 0 TD (tigations:)Tj /F4 12 Tf -475.224 -29 TD (T)Tj 5.008 0 TD (o In)Tj 21.391 0 TD (oc)Tj 12.854 0 TD (ul)Tj 12.259 0 TD [ (a)63 (t)21 (e)-250 (V)21 (e)21 (get)] TJ 47.993 0 TD (ab)Tj 12.968 0 TD (l)Tj 4.716 0 TD (e S)Tj 15.648 0 TD [ (li)21 (ces)] TJ /F3 12 Tf -132.743 -14.25 TD [ (W)42 (a)] TJ 15.338 0 TD (sh potato tubers, carrots, onions, and cut)Tj 214.176 0 TD (into slic)Tj 39.874 0 TD [ (e)-21 (s)] TJ 14.78 0 TD (about)Tj 32.337 0 TD (7mm \(1/4 inch\) thick. Put)Tj 133.419 0 TD (about)Tj 32.337 0 TD (1/8 inc)Tj 35.197 0 TD (h)Tj -517.556 -14.5 TD (of)Tj 12.767 0 TD [ (s)-21 (t)] TJ 9.513 0 TD (eri)Tj 13.339 0 TD (l)Tj 3.167 0 TD 0.187 Tw (e water in a Petri dish that)Tj 0 Tw 136.906 0 TD (c)Tj 5.185 0 TD (ontain)Tj 32.372 0 TD (s)Tj 8.687 0 TD (two disk)Tj 42.646 0 TD (s)Tj 8.687 0 TD (of)Tj 12.767 0 TD [ (s)-21 (t)] TJ 9.513 0 TD (eri)Tj 13.339 0 TD (l)Tj 2.917 0 TD 0.27 Tw (e filter paper and a)Tj 0 Tw 96.777 0 TD (d)Tj 6.659 0 TD (d the )Tj 29.21 0 TD [ (ve)21 (g)] TJ 17.706 0 TD (etab)Tj 22.508 0 TD (l)Tj 2.917 0 TD (e slic)Tj 26.279 0 TD (e)Tj 6.094 0 TD (s.)Tj -519.93 -14.25 TD (M)Tj 9.586 0 TD (ake a slight)Tj 61.88 0 TD (c)Tj 5.435 0 TD (ut)Tj 13.08 0 TD (in the center of)Tj 79.76 0 TD (e)Tj 6.094 0 TD (ach slice, and inoc)Tj 94.593 0 TD (ul)Tj 9.421 0 TD (ate with a loopf)Tj 79.172 0 TD [ (u)21 (l)] TJ 12.421 0 TD (of)Tj 13.017 0 TD (c)Tj 5.185 0 TD (el)Tj 9.011 0 TD (ls)Tj 11.604 0 TD (from the c)Tj 51.619 0 TD (u)Tj 6.254 0 TD (lture.)Tj -467.959 -14.5 TD (Include a non-inoc)Tj 94.075 0 TD (ul)Tj 9.671 0 TD (ated slice for each tr)Tj 103.544 0 TD (e)Tj 5.844 0 TD (atment)Tj 38.979 0 TD (u)Tj 6.254 0 TD (sed. Seal)Tj 52.919 0 TD [ (p)21 (l)] TJ 9.314 0 TD (at)Tj 9.993 0 TD [ (es)-229 (w)] TJ 22.845 0 TD (ith strip)Tj 39.823 0 TD (s)Tj 8.687 0 TD (of)Tj 12.517 0 TD (Saran )Tj 32.616 0 TD (Wr)Tj 13.237 0 TD (ap or Parafilm.)Tj /F4 12 Tf -460.458 -28.75 TD (T)Tj 5.008 0 TD (o In)Tj 21.391 0 TD (oc)Tj 12.854 0 TD (ul)Tj 12.259 0 TD [ (a)63 (t)21 (e)-250 (W)] TJ 28.561 0 TD (ho)Tj 14.613 0 TD [ (l)21 (e)-250 (V)21 (e)21 (get)] TJ 42.313 0 TD (ab)Tj 12.718 0 TD [ (le)21 (s)] TJ /F3 12 Tf -149.577 -14.5 TD (C)Tj 6.338 0 TD (aref)Tj 20.175 0 TD [ (u)21 (l)] TJ 9.421 0 TD (ly)Tj 11.545 0 TD (wa)Tj 14.231 0 TD (sh the sur)Tj 51.188 0 TD (f)Tj 3.598 0 TD (ace of)Tj 33.18 0 TD (who)Tj 21.178 0 TD (l)Tj 3.417 0 TD 0.131 Tw (e potato tubers, carrots, and onions. Sterilize a t)Tj 0 Tw 249.495 0 TD [ (e)21 (as)] TJ 17.697 0 TD (ing needle b)Tj 63.044 0 TD (y)Tj -504.622 -14.25 TD (dipping in 95% ethano)Tj 115.901 0 TD (l)Tj 5.917 0 TD (and flaming unti)Tj 83.348 0 TD [ (l)-250 (r)] TJ 10.233 0 TD (ed-hot. Let)Tj 60.776 0 TD (the needle coo)Tj 75.196 0 TD (l)Tj 5.917 0 TD (\(thi)Tj 17.238 0 TD [ (s)-229 (is)-229 (v)] TJ 25.545 0 TD (er)Tj 10.16 0 TD (y)Tj 8.628 0 TD (impor)Tj 29.862 0 TD (t)Tj 3.826 0 TD (ant\), dip into a)Tj -452.502 -14.5 TD [ (c)21 (u)] TJ 11.688 0 TD (lture of)Tj 39.392 0 TD (Er)Tj 10.078 0 TD (w)Tj 8.314 0 TD (inia carot)Tj 47.421 0 TD (ov)Tj 11.912 0 TD (ora and then s)Tj 73.465 0 TD (t)Tj 3.826 0 TD (ab the potato 4-5 time)Tj 113.18 0 TD [ (s)-229 (w)] TJ 16.751 0 TD (ith the needle. Repeat)Tj 118.192 0 TD (thi)Tj 13.449 0 TD (s)Tj 8.687 0 TD (2-3 time)Tj 41.721 0 TD (s)Tj 8.437 0 TD (in)Tj -526.556 -14.5 TD (the same ar)Tj 60.032 0 TD (e)Tj 6.094 0 TD (a of)Tj 21.902 0 TD (the potat)Tj 46.218 0 TD (o)Tj 6.419 0 TD (. F)Tj 14.276 0 TD [ (o)-21 (l)] TJ 9.586 0 TD (low the same procedure for the carrot)Tj 194.482 0 TD 0.22 Tw (and the onion. For contr)Tj 0 Tw 124.681 0 TD (ol)Tj 9.586 0 TD (s, use)Tj -493.119 -14.25 TD (the e)Tj 25.426 0 TD (x)Tj 5.663 0 TD (act)Tj 18.178 0 TD (procedure, e)Tj 63.989 0 TD (xc)Tj 10.848 0 TD (ept)Tj 19.317 0 TD (dip the t)Tj 42.862 0 TD [ (e)21 (as)] TJ 17.697 0 TD (ing needle into s)Tj 84.967 0 TD (t)Tj 3.576 0 TD (eri)Tj 13.339 0 TD (l)Tj 2.917 0 TD (e water \(in)Tj 53.481 0 TD [ (s)-21 (t)21 (ea)] TJ 21.523 0 TD (d of)Tj 22.644 0 TD (the bacterial)Tj 66.854 0 TD (cu)Tj 11.938 0 TD (lture\))Tj -485.292 -14.5 TD (prior to s)Tj 45.996 0 TD (t)Tj 3.826 0 TD (abbing the )Tj 57.873 0 TD (veg)Tj 17.706 0 TD (etab)Tj 22.508 0 TD (le)Tj 9.011 0 TD (s. Place inoc)Tj 66.412 0 TD (ul)Tj 9.671 0 TD (ated )Tj 25.464 0 TD (veg)Tj 17.706 0 TD (etab)Tj 22.508 0 TD (les)Tj 17.697 0 TD (onto a wet)Tj 56.267 0 TD (p)Tj 6.647 0 TD (aper t)Tj 29.769 0 TD (ow)Tj 14.733 0 TD (el)Tj 12.011 0 TD (in)Tj 9.444 0 TD (s)Tj 5.687 0 TD (ide a p)Tj 34.432 0 TD [ (la)21 (s)] TJ 14.771 0 TD (tic)Tj 15.189 0 TD (bag)Tj -515.293 -14.25 TD (and seal)Tj 46.174 0 TD (tightly)Tj 35.439 0 TD (w)Tj 8.314 0 TD (ith a tw)Tj 37.693 0 TD [ (i)21 (s)-21 (t)] TJ 15.191 0 TD 0.27 Tw (tie. Use a separate b)Tj 0 Tw 107.924 0 TD (a)Tj 6.167 0 TD (g for eac)Tj 43.584 0 TD [ (h)-229 (veg)] TJ 27.15 0 TD (etab)Tj 22.508 0 TD (l)Tj 3.167 0 TD (e and separate b)Tj 85.509 0 TD [ (ag)21 (s)-229 (f)] TJ 24.57 0 TD (or the)Tj -463.432 -14.5 TD (c)Tj 5.185 0 TD (ontr)Tj 20.827 0 TD [ (ol)21 (s)] TJ 18.022 0 TD (\(ver)Tj 19.192 0 TD (y)Tj 8.378 0 TD (impor)Tj 30.112 0 TD (t)Tj 3.826 0 TD (ant\).)Tj ET endstream endobj 36 0 obj 16669 endobj 34 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F2 9 0 R /F3 11 0 R /F4 15 0 R >> /ProcSet 2 0 R >> /Contents 35 0 R >> endobj 39 0 obj << /Length 40 0 R >> stream 0.25 w 1 g 36.25 36.75 539 719 re f BT /F4 12 Tf 0 Tc 0 Tw 0 g 37.405 745.25 TD [ (T)42 (o)-250 (T)21 (es)63 (t)-250 (T)42 (e)21 (m)] TJ 59.106 0 TD (pe)Tj 11.528 0 TD [ (r)21 (a)83 (tur)21 (e)-271 (Ef)21 (f)21 (e)] TJ 59.665 0 TD (ct)Tj 10.956 0 TD [ (s)-229 (o)] TJ 16.579 0 TD (n S)Tj 17.244 0 TD [ (o)21 (f)21 (t)] TJ 20.412 0 TD (Ro)Tj 13.68 0 TD (t)Tj /F3 12 Tf -209.186 -14.25 TD (Inoc)Tj 21.583 0 TD (ul)Tj 9.421 0 TD (ate the ti)Tj 45.392 0 TD (ss)Tj 11.373 0 TD (ue slic)Tj 32.783 0 TD [ (e)21 (s)] TJ 14.53 0 TD (of)Tj 12.767 0 TD (who)Tj 21.428 0 TD [ (l)21 (e)-250 (ve)21 (g)] TJ 29.717 0 TD (etab)Tj 22.508 0 TD [ (l)21 (es)] TJ 17.697 0 TD (in a refrigerator, at)Tj 98.212 0 TD (r)Tj 4.066 0 TD (oom temperature, at)Tj 107.196 0 TD (26 degree)Tj 51.601 0 TD (s)Tj 8.437 0 TD (C,)Tj -508.72 -14.5 TD (and 45 degree)Tj 72.92 0 TD [ (s)-250 (C)] TJ 18.024 0 TD (if)Tj 9.776 0 TD (incubator)Tj 48.532 0 TD (s)Tj 8.437 0 TD (are avai)Tj 40.051 0 TD (l)Tj 2.917 0 TD (ab)Tj 12.838 0 TD (l)Tj 3.167 0 TD (e. Different)Tj 62.56 0 TD (group)Tj 29.756 0 TD (s)Tj 8.437 0 TD (of)Tj 12.767 0 TD [ (s)-21 (t)] TJ 9.513 0 TD (udents)Tj 38.035 0 TD (c)Tj 5.185 0 TD (an choose one temperature.)Tj /F4 12 Tf -382.818 -28.75 TD (T)Tj 5.008 0 TD (o De)Tj 23.816 0 TD (ter)Tj 16.81 0 TD (min)Tj 19.389 0 TD [ (e)-271 (E)21 (n)] TJ 21.99 0 TD (zy)Tj 10.416 0 TD [ (me)-250 (A)] TJ 24.211 0 TD (ctivit)Tj 29.417 0 TD [ (y)-250 (an)] TJ 21.356 0 TD (d S)Tj 17.004 0 TD (oft)Tj 20.662 0 TD (Ro)Tj 13.93 0 TD (t)Tj 8.064 0 TD (De)Tj 13.304 0 TD [ (vel)42 (o)21 (p)21 (m)] TJ 37.728 0 TD (en)Tj 12.526 0 TD (t)Tj /F3 12 Tf -295.686 -14.5 TD (The inoc)Tj 42.609 0 TD (ul)Tj 9.671 0 TD (ated ti)Tj 32.469 0 TD (ss)Tj 11.123 0 TD (ue slic)Tj 32.783 0 TD (es)Tj 14.53 0 TD (shou)Tj 25.304 0 TD (l)Tj 3.167 0 TD (d be chec)Tj 48.267 0 TD (k)Tj 6.094 0 TD (ed s)Tj 21.158 0 TD (t)Tj 3.826 0 TD (arting at)Tj 45.834 0 TD (48 hour)Tj 40.449 0 TD (s)Tj 8.437 0 TD (wher)Tj 24.919 0 TD [ (ea)21 (s)] TJ 20.447 0 TD (the who)Tj 41.01 0 TD [ (l)-21 (e)-208 (veg)] TJ 29.967 0 TD (etab)Tj 22.508 0 TD [ (le)21 (s)] TJ -484.553 -14.25 TD (shou)Tj 25.304 0 TD (l)Tj 2.917 0 TD (d be chec)Tj 48.517 0 TD (k)Tj 5.594 0 TD (ed beginning at)Tj 83.089 0 TD (five d)Tj 27.741 0 TD (ays)Tj 20.231 0 TD (af)Tj 9.765 0 TD (t)Tj 3.826 0 TD (er inoc)Tj 34.676 0 TD [ (u)21 (l)] TJ 9.421 0 TD 0.27 Tw (ation. For the ti)Tj 0 Tw 80.423 0 TD (ss)Tj 11.123 0 TD (ue slic)Tj 32.783 0 TD (e)Tj 6.094 0 TD (s, t)Tj 15.923 0 TD [ (es)21 (t)-250 (f)] TJ 21.704 0 TD (or ti)Tj 20.208 0 TD [ (s)-21 (s)] TJ 11.373 0 TD (ue)Tj -470.652 -14.5 TD (m)Tj 9.301 0 TD (aceration \(sof)Tj 69.828 0 TD [ (t)-250 (r)] TJ 10.643 0 TD (ot\) b)Tj 23.435 0 TD (y)Tj 8.378 0 TD (running a transfer loop evenly)Tj 155.617 0 TD [ (o)21 (v)] TJ 11.662 0 TD (er the slice and/or b)Tj 103.831 0 TD (y)Tj 8.128 0 TD (pr)Tj 10.714 0 TD [ (o)-21 (b)] TJ 13.09 0 TD (ing with a t)Tj 56.725 0 TD [ (ea)21 (s)] TJ 17.447 0 TD (in)Tj 9.944 0 TD (g)Tj -508.881 -14.5 TD 0.198 Tw (needle. Do the same for the inoc)Tj 0 Tw 168.487 0 TD (ul)Tj 9.421 0 TD (ated ar)Tj 36.198 0 TD (e)Tj 5.844 0 TD (a on who)Tj 46.215 0 TD [ (l)-21 (e)-208 (veg)] TJ 29.967 0 TD (etab)Tj 22.508 0 TD [ (le)21 (s)] TJ 17.697 0 TD (\(be sure to use separate transfer loop)Tj 191.412 0 TD (s)Tj -527.813 -14.25 TD (or t)Tj 17.28 0 TD [ (ea)21 (s)] TJ 17.447 0 TD (ing needl)Tj 47.561 0 TD [ (es)-229 (f)] TJ 18.128 0 TD (or inoc)Tj 35.001 0 TD (ul)Tj 9.421 0 TD (ated ti)Tj 32.469 0 TD (ss)Tj 11.373 0 TD (ue)Tj 12.348 0 TD (s)Tj 8.437 0 TD (and contr)Tj 48.572 0 TD (ol)Tj 9.336 0 TD (s!\). Continue to e)Tj 89.592 0 TD (x)Tj 5.663 0 TD (amine for 2-3 d)Tj 77.256 0 TD [ (a)21 (ys)] TJ 19.981 0 TD (and note the)Tj -459.753 -14.5 TD (difference in rot)Tj 83.773 0 TD (produced on the different)Tj 132.815 0 TD [ (ve)21 (g)] TJ 17.706 0 TD (etab)Tj 22.508 0 TD (les)Tj 17.697 0 TD (and between the different)Tj 134.598 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.671 0 TD (ation)Tj -440.394 -14.25 TD (\(temperature\) conditions. Note the odor produced b)Tj 265.987 0 TD (y)Tj 8.628 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.421 0 TD (ated, rotting ti)Tj 73.081 0 TD (ss)Tj 11.123 0 TD (ue. Note: If)Tj 61.942 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.421 0 TD (ated)Tj -482.754 -14.5 TD (veg)Tj 17.956 0 TD (etab)Tj 22.508 0 TD [ (l)21 (es)] TJ 17.697 0 TD (in the b)Tj 38.666 0 TD (ags)Tj 20.973 0 TD (are incubated for appr)Tj 113.643 0 TD [ (o)42 (x)] TJ 11.582 0 TD (imately)Tj 40.361 0 TD (two week)Tj 47.623 0 TD (s)Tj 8.687 0 TD (at)Tj 12.993 0 TD (r)Tj 3.816 0 TD (oom temperature or 26 degree)Tj 155.815 0 TD [ (s)-229 (C)] TJ -512.226 -14.5 TD (they)Tj 24.992 0 TD [ (wil)21 (l)] TJ 20.577 0 TD (\322melt\323 and become almo)Tj 127.083 0 TD [ (s)-21 (t)-208 (t)] TJ 16.089 0 TD (otally)Tj 31.124 0 TD (liquefied due to the gr)Tj 113.294 0 TD (e)Tj 6.094 0 TD (at)Tj 12.743 0 TD (amount)Tj 41.732 0 TD (of)Tj 12.767 0 TD (pectic)Tj 33.615 0 TD (enzyme activity)Tj 78.279 0 TD (.)Tj /F4 12 Tf -518.366 -28.75 TD (T)Tj 5.008 0 TD (o De)Tj 24.066 0 TD [ (m)21 (on)21 (s)] TJ 28.525 0 TD (tr)Tj 11.22 0 TD [ (a)83 (t)] TJ 10.396 0 TD (e K)Tj 15.36 0 TD [ (o)21 (c)] TJ 12.854 0 TD (h\325s)Tj 19.636 0 TD [ (P)42 (o)-21 (s)63 (t)] TJ 24.72 0 TD (ul)Tj 12.259 0 TD [ (a)83 (t)21 (es)] TJ /F3 12 Tf -164.146 -14.25 TD (Af)Tj 10.662 0 TD (t)Tj 3.576 0 TD (er s)Tj 18.815 0 TD (t)Tj 3.826 0 TD (udents)Tj 38.035 0 TD (h)Tj 6.694 0 TD (ave o)Tj 26.891 0 TD (b)Tj 6.671 0 TD (ser)Tj 15.847 0 TD (v)Tj 5.493 0 TD (ed the \322con)Tj 58.989 0 TD (st)Tj 9.263 0 TD (ant)Tj 19.259 0 TD [ (a)-21 (s)] TJ 11.854 0 TD (soc)Tj 17.54 0 TD (i)Tj 2.929 0 TD (ation\323 between ti)Tj 87.972 0 TD [ (s)21 (s)] TJ 11.373 0 TD (ue)Tj 12.348 0 TD (s)Tj 8.437 0 TD (inoc)Tj 21.548 0 TD (ul)Tj 9.671 0 TD (ated with Er)Tj 60.774 0 TD (w)Tj 8.064 0 TD (ini)Tj 12.873 0 TD (a)Tj -489.333 -14.5 TD (c)Tj 5.185 0 TD (arot)Tj 20.229 0 TD [ (o)-21 (v)] TJ 11.912 0 TD (ora and sof)Tj 57.383 0 TD [ (t)-250 (r)] TJ 10.893 0 TD (ot)Tj 12.995 0 TD (symptoms, have them iso)Tj 130.989 0 TD (l)Tj 2.917 0 TD (ate the bacterium \(u)Tj 102.891 0 TD (s)Tj 5.687 0 TD (ing a s)Tj 33.603 0 TD (t)Tj 3.826 0 TD (eri)Tj 13.339 0 TD (l)Tj 2.917 0 TD (e transfer loop\) int)Tj 94.491 0 TD (o)Tj -509.331 -14.5 TD (p)Tj 6.647 0 TD (ure c)Tj 25.067 0 TD (u)Tj 6.504 0 TD (lture on a nutrient)Tj 94.689 0 TD (ag)Tj 12.536 0 TD (ar p)Tj 19.599 0 TD (l)Tj 3.167 0 TD (ate \(compare with the pure c)Tj 145.469 0 TD (u)Tj 6.504 0 TD (lture used for the original)Tj 132.732 0 TD (inoc)Tj 21.548 0 TD [ (u)21 (l)] TJ 9.421 0 TD (ations\).)Tj -483.772 -14.25 TD (Af)Tj 10.412 0 TD (t)Tj 3.826 0 TD (er the pure c)Tj 64.175 0 TD (u)Tj 6.504 0 TD (lture h)Tj 33.07 0 TD [ (a)21 (s)] TJ 14.604 0 TD (gr)Tj 10.436 0 TD (o)Tj 6.169 0 TD (wn, use it)Tj 52.248 0 TD (t)Tj 3.826 0 TD (o inoc)Tj 30.935 0 TD [ (u)21 (l)] TJ 9.421 0 TD (at)Tj 9.743 0 TD [ (e)-250 (veg)] TJ 26.8 0 TD (etab)Tj 22.508 0 TD [ (les)-229 (by)] TJ 32.746 0 TD (the procedure de)Tj 86.788 0 TD (sc)Tj 11.121 0 TD (ribed abo)Tj 48.644 0 TD (v)Tj 5.493 0 TD (e \(u)Tj 19.105 0 TD (s)Tj 5.437 0 TD (in)Tj 9.694 0 TD (g)Tj -523.631 -14.5 TD (the same )Tj 49.548 0 TD (veg)Tj 17.956 0 TD (etab)Tj 22.508 0 TD (l)Tj 2.917 0 TD (e sour)Tj 31.738 0 TD (c)Tj 5.185 0 TD (e\), o)Tj 22.224 0 TD (b)Tj 6.921 0 TD (ser)Tj 15.847 0 TD (v)Tj 5.493 0 TD (e the symptoms)Tj 84.389 0 TD (produced and then re-iso)Tj 128.439 0 TD (l)Tj 2.917 0 TD 0.27 Tw (ate the bacterium in pur)Tj 0 Tw 122.728 0 TD (e)Tj -518.906 -14.25 TD [ (c)21 (u)] TJ 11.688 0 TD (lture \(compare with original)Tj 143.805 0 TD [ (c)21 (u)] TJ 11.688 0 TD (lture used to inoc)Tj 89.048 0 TD (ul)Tj 9.421 0 TD (ate these )Tj 50.418 0 TD [ (v)21 (e)21 (g)] TJ 17.456 0 TD (etab)Tj 22.508 0 TD (le)Tj 9.261 0 TD (s.)Tj /F2 12 Tf -365.18 -29 TD (Qu)Tj 15.298 0 TD (es)Tj 11.403 0 TD (tio)Tj 16.244 0 TD (ns)Tj /F3 12 Tf -42.965 -14.25 TD (1. Wh)Tj 27.53 0 TD (y)Tj 8.378 0 TD (are pectic)Tj 52.66 0 TD (polymer)Tj 41.572 0 TD (s)Tj 8.437 0 TD (impor)Tj 30.112 0 TD (t)Tj 3.826 0 TD (ant)Tj 19.259 0 TD (in p)Tj 19.06 0 TD (l)Tj 3.417 0 TD (ants)Tj 24.945 0 TD (and what)Tj 50.062 0 TD [ (i)21 (s)] TJ 11.365 0 TD (their f)Tj 30.425 0 TD (u)Tj 6.254 0 TD (nction?)Tj -337.369 -14.5 TD (2. How doe)Tj 56.991 0 TD (s)Tj 8.687 0 TD (Er)Tj 10.078 0 TD (w)Tj 8.314 0 TD (inia carot)Tj 47.421 0 TD (ov)Tj 11.662 0 TD (ora utilize the \322dig)Tj 94.839 0 TD [ (e)-21 (s)21 (t)] TJ 15.606 0 TD (ed\323 p)Tj 27.935 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.259 0 TD (ti)Tj 7.005 0 TD [ (s)21 (s)] TJ 11.373 0 TD (ue a)Tj 21.483 0 TD (s)Tj 8.437 0 TD (a food sour)Tj 57.624 0 TD (c)Tj 5.435 0 TD (e?)Tj -415.165 -14.25 TD (3. Do other p)Tj 66.083 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.509 0 TD (p)Tj 6.647 0 TD (athogen)Tj 41.585 0 TD [ (s)-271 (s)] TJ 14.373 0 TD (uch a)Tj 27.268 0 TD [ (s)-250 (fu)21 (n)] TJ 25.054 0 TD (gi and )Tj 34.576 0 TD (v)Tj 5.243 0 TD (iruse)Tj 25.529 0 TD (s)Tj 8.437 0 TD (produce cel)Tj 58.988 0 TD [ (l)-229 (w)] TJ 14.231 0 TD (all-degr)Tj 38.825 0 TD (a)Tj 6.417 0 TD (ding enzymes?)Tj -395.553 -14.5 TD (4. What)Tj 41.846 0 TD (was)Tj 22.918 0 TD (the effect)Tj 50.976 0 TD (of)Tj 12.767 0 TD (t)Tj 3.576 0 TD (emperature on the rate of)Tj 133.45 0 TD (the sof)Tj 35.285 0 TD [ (t)-229 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD (e)Tj 6.094 0 TD (xperiment? Why?)Tj -330.671 -14.5 TD (5. What)Tj 41.34 0 TD (do the p)Tj 42.276 0 TD (l)Tj 2.917 0 TD (ant)Tj 19.259 0 TD (m)Tj 9.551 0 TD (aterial)Tj 32.416 0 TD (s)Tj 8.687 0 TD (\(v)Tj 8.782 0 TD (eg)Tj 12.463 0 TD (etab)Tj 22.508 0 TD (le)Tj 9.011 0 TD (s\) used in thi)Tj 66.478 0 TD [ (s)-229 (e)] TJ 14.28 0 TD (xperiment)Tj 54.636 0 TD (al)Tj 9.334 0 TD [ (l)-229 (h)] TJ 12.361 0 TD (ave in common?)Tj -366.235 -14.25 TD [ (6)21 (.)] TJ 12.875 0 TD (Wh)Tj 16.365 0 TD (y)Tj 8.628 0 TD (did the sliced ti)Tj 78.74 0 TD [ (s)21 (s)] TJ 11.123 0 TD (ue)Tj 12.598 0 TD (s)Tj 8.437 0 TD (develop sof)Tj 59.244 0 TD [ (t)-250 (r)] TJ 10.643 0 TD (ot)Tj 12.995 0 TD [ (fa)21 (st)] TJ 18.777 0 TD (er than the who)Tj 80.31 0 TD [ (l)21 (e)-250 (veg)] TJ 29.717 0 TD (etab)Tj 22.508 0 TD (l)Tj 3.167 0 TD (e?)Tj -386.165 -14.5 TD [ (7)104 (.)] TJ 10.905 0 TD (What)Tj 29.108 0 TD [ (i)21 (s)] TJ 11.615 0 TD (the impor)Tj 49.445 0 TD (t)Tj 3.826 0 TD (ance of)Tj 39.696 0 TD (K)Tj 6.814 0 TD (och\325s)Tj 29.566 0 TD (po)Tj 13.066 0 TD [ (stu)21 (l)] TJ 18.934 0 TD (at)Tj 9.743 0 TD (e)Tj 6.094 0 TD (s?)Tj ET q 163.25 0 0 53.75 409 37.25 cm /I2 Do endstream endobj 40 0 obj 10461 endobj 41 0 obj << /Type /XObject /Subtype /Image /Name /I2 /Filter /DCTDecode /Width 682 /Height 225 /BitsPerComponent 8 /ColorSpace /DeviceRGB /Length 42 0 R >> stream AdobedC+ "+6.++(:SRB3BMPef`bb`knuruvh}  a!1AQa"q2B#$Rb34rC%Scs5&DTdE£t6UeuF'Vfv7GWgw(8HXhx )9IYiy *:JZjz ?F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$HIHwSy>úO0;5!޲±ⵁκ.ϱ՗:ðA+ <WiXS aWiXS aWiXS a*EAAv(Ue,"#Gʫ.edĐAAF4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F5Oٙm5`y§l0FSf`[Mn#^`,ïhkpէ Y^9cwO} L+r視Vj_Nَ&}:f;HFꟽ"a=ZoKp68zޖmݷq+k-ۻoxU&GY{a '&GY{a '&GY{a 'qۺUqۺUqۺP?kid~黃~&9@wLr_ela{'q:.Vw_ela{'q:(ci0 ;L†>N>H&[`3$F4I$_F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$ӹ21Q;qvW2qW[=:'{9.~JN8pǺUDg!Oyس^U.e6w|Gc"/;kʥן̲VebyTPC/c͹{@mM vC/c͹{@mM vC/c͹{@mM v[N^m[s7;EF_U/6ڭӝ/*ӗmVQ/{~%hy=I?K<㇞y$SoJqkkt8|ҺyZ>Zs WȤi5deau-9 ȃVFVYܓ[>immPeS PeS PeS ַl}o4XdN[p>Ʒ,2'Py8~[c[yZXUek:A,Lm V&UYt6·yi($F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I"@xH?nOQ~}GY{'s-9o61wc@0\.e=G5mf5 h B+|۔?Z[ܦX<qШu 62+W }m¶_aEeAʃyͿg}?%[eonWe`y~K62ݞ;`:>95)贱)ɬ۰ڵѲ^/H. q=|=LH>*q==L>*q==L>*LηɯU>0ձņT|ʡAT cj#ϼ2P©k{{OF_soU9p/ob;pmC 7G}?C]m89&jps\']>Mw1O p p pܨPpcw[* `n\c+rEA Kw%G':j]mkOQΧ[irsƥv}GP6ak$N>CGP6ak$N>CGP6ak$N>CܜfXgp5ܜfXgp5ܜfXgp5ѹgR%2 ewbd2ԪoYhث u*[ Z;v*>'Q9k*lqUKǵ6 ]Q󪥏zc ylL7ʚK Ēpf&eM%\I[8}3 .q$jڶF4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$W]cكvp%w?Z}J{0q.x O]uf9e?O)m42|J54Sil>djh |*3%Fd̔JȔJȔJȕշ*슞U@x{WdTLzămʻ":c$%VmٖqiZzͷfZ!Ŧ$xwUiO6ݙkpZt>RlZt>RlZt>RlYocxlh1V7ƁYocxlhվ]P~;zB]P~;zB]P~;zBI8 ,nM7JI8 ,nM7JI8 ,nM7Jg_iN U :KH u2YZ@mu0|*ye@XxWnO[,urgh rzYc+;@0>+szǧjۀii$N;S>II'wYnݧ|Vѝcq\)npuwgdXW n;]nm7›.lAn&w0G=V×W-dHrrLZ{I+*ךKƺ!}BҭyԽkW{O+*ךKƺ!}BF4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4I$I$F4Ieu_^ii}.gdrdm~}yuVWQ斗zVG-AFT.#h~iz57?"qCkOfѩ Z{7޿ZY9mדG'8vwh$^N;䭵@ֆ Z4 YI\ccu# YI\ccu# YI\ccu#/F4#H{FLJ5^<8@fwP'oNqӱЫȗ8R0XUK)y忘Ȭgt*%?AU6:8yQmh,3AU6:8ySH-h M %66v[u4ؖoRƳ5knkL-f,k3_vpoRƳ5knkL!Ǵ_ 3落c;z}!} xboO~0ϾaT.*I_Wu`SuoM?:  ëi"Q԰V!ǀ[? 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